Abstract

We present a new concept for fluorescence lifetime imaging (FLIM) based on time-resolved Hadamard imaging (HI). HI allows image acquisition by use of one single-point detector without requiring a moving scanning stage. Moreover, it reduces the influence of detector noise compared with raster scanning. By use of Monte Carlo simulations it could be confirmed that Hadamard transformation may decrease the error in lifetime estimation and in general in fluorescence parameter estimation when the signal-to-noise ratio is low and detector dark noise is high. This concept may find applications whenever the performance of FLIM or similar methods is limited by high dark-count rates and when the use of a single-point detector is preferable.

© 2005 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Penalized maximum likelihood estimation of lifetime and amplitude images from multi-exponentially decaying fluorescence signals

Jeongtae Kim, Jiyeong Seok, Hwiin Lee, and Minyung Lee
Opt. Express 21(17) 20240-20253 (2013)

Hadamard multiplexed fluorescence tomography

Ali Behrooz, Ali A. Eftekhar, and Ali Adibi
Biomed. Opt. Express 5(3) 763-777 (2014)

On-chip, time-correlated, fluorescence lifetime extraction algorithms and error analysis

Day-Uei Li, Eleanor Bonnist, David Renshaw, and Robert Henderson
J. Opt. Soc. Am. A 25(5) 1190-1198 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (12)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (41)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription