Effects of index-mismatch-induced spherical aberration in pump–probe microscopic image formation

Peter T. Fwu; Po-Hsiang Wang; Chi-Kuan Tung; Chen-Yuan Dong

doi:10.1364/AO.44.004220

Applied Optics
Vol. 44,
Issue 20,
pp. 4220-4227
(2005)
•https://doi.org/10.1364/AO.44.004220

Effects of index-mismatch-induced spherical aberration in pump–probe microscopic image formation

Peter T. Fwu, Po-Hsiang Wang, Chi-Kuan Tung, and Chen-Yuan Dong

Not Accessible

Your library or personal account may give you access

Get PDF
Email
Share
Get Citation
Copy Citation Text
Peter T. Fwu, Po-Hsiang Wang, Chi-Kuan Tung, and Chen-Yuan Dong, "Effects of index-mismatch-induced spherical aberration in pump–probe microscopic image formation," Appl. Opt. 44, 4220-4227 (2005)

Export Citation
- BibTex
- Endnote (RIS)
- HTML
- Plain Text
Citation alert
Save article

Abstract

Pump–probe fluorescence microscopy has been demonstrated to be a powerful tool for obtaining three-dimensional, time-resolved information in bioimaging applications. However, the use of this technique can be complicated by the fact that the different wavelengths used to achieve pump–probe microscopy can result in wavelength-dependent spherical aberration, thus limiting the usefulness of the technique. We address this issue by investigating the effects of refractive-index-mismatch-induced spherical aberration on pump–probe image formation. We model the effects by considering pump–probe imaging performed with an objective with a numerical aperture of 0.75 focusing through an oil–water interface. Our results show that spherical aberration has the greatest effect in degrading an axial point-spread function. In addition to signal loss, the redistribution of signal strength along the axial direction results in broadening of the FWHM of the plane response function. The inclusion of confocal detection tends to improve image resolution but at a significant loss of signal strength.

Full Article | PDF Article

More Like This

Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens

Cees J. de Grauw, Jurrien M. Vroom, Hans T. M van der Voort, and Hans C. Gerritsen
Appl. Opt. 38(28) 5995-6003 (1999)

Zeeman laser scanning confocal microscope and its ability on reduction of specimen-induced spherical aberration

Jheng-Syong Wu, Chien Chou, Chi-Hui Chang, Li-Ping Yu, Li-Dek Chou, Hsiu-Fong Chang, Hon-Fai Yau, and Cheng-Chung Lee
Opt. Express 18(12) 13136-13150 (2010)

Effect of spherical aberration on the imaging properties of scanning optical microscopes

C. J. R. Sheppard and T. Wilson
Appl. Opt. 18(7) 1058-1063 (1979)

Previous Article Next Article

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Depth (µm)	Excitation Mode	Focal Shift (µm)	Maximum N.I.	Axial-FWHM (PSF) (µm)	Radial-FWHM (PSF) (µm)	Normalized Signal Level	Axial-P-FWHM (µm)
1pu–1pr; λ_pump, 350 nm; λ_probe, 350 nm; λ_confocal, 450 nm
0	1pu–1pr	0	1	1.129	0.174	1	1.193
	1pu–1pr Conf	0	1	0.992	0.152	1	1.004
50	1pu–1pr	8.229	0.548	1.203	0.174	0.749	1.394
	1pu–1pr Conf	8.240	0.457	1.057	0.153	0.523	1.083
100	1pu–1pr	14.902	0.085	3.175	0.230	0.488	3.554
	1pu–1pr Conf	16.313	0.040	2.408	0.162	0.137	2.578
150	1pu–1pr	23.046	0.066	1.845	0.207	0.384	3.150
	1pu–1pr Conf	22.935	0.019	1.615	0.187	0.075	1.982
200	1pu–1pr	29.624	0.039	1.950	0.231	0.317	3.517
	1pu–1pr Conf	29.789	0.007	2.130	0.207	0.044	2.443
1pu–1pr; λ_pump, 350 nm; λ_probe, 500 nm; λ_confocal, 450 nm
0	1pu–1pr	0	1	1.300	0.199	1	1.426
	1pu–1pr Conf	0	1	1.107	0.170	1	1.125
50	1pu–1pr	8.232	0.640	1.379	0.200	0.791	1.572
	1pu–1pr Conf	8.241	0.534	1.158	0.170	0.594	1.196
100	1pu–1pr	16.348	0.158	2.466	0.210	0.505	3.112
	1pu–1pr Conf	16.369	0.075	1.673	0.176	0.172	1.991
150	1pu–1pr	22.910	0.073	2.688	0.251	0.390	3.053
	1pu–1pr Conf	22.801	0.022	2.031	0.216	0.087	2.124
200	1pu–1pr	30.000	0.047	2.717	0.263	0.322	3.731
	1pu–1pr Conf	30.236	0.010	2.406	0.222	0.054	2.751
2pu; λ_excitation, 700 nm; λ_confocal, 450 nm
0	2pu	0	1	2.259	0.347	1	2.385
	2pu Conf	0	1	1.517	0.234	1	1.566
50	2pu	8.247	0.862	2.288	0.346	0.922	2.462
	2pu Conf	8.248	0.720	1.560	0.233	0.763	1.619
100	2pu	16.458	0.548	2.413	0.349	0.747	2.787
	2pu Conf	16.454	0.260	1.777	0.237	0.358	1.898
150	2pu	24.633	0.251	2.811	0.352	0.585	4.226
	2pu Conf	24.308	0.048	3.326	0.255	0.148	3.137
200	2pu	29.816	0.085	9.428	0.462	0.486	7.109
	2pu Conf	30.588	0.019	3.821	0.300	0.093	3.523
2pu–1pr; λ_pump, 700 nm; λ_probe, 350 nm; λ_confocal, 450 nm
0	2pu–1pr	0	1	1.300	0.200	1	1.404
	2pu–1pr Conf	0	1	1.108	0.170	1	1.123
50	2pu–1pr	8.236	0.638	1.369	0.201	0.719	1.502
	2pu–1pr Conf	8.226	0.533	1.161	0.171	0.581	1.184
100	2pu–1pr	16.376	0.157	2.072	0.207	0.299	2.088
	2pu–1pr Conf	16.402	0.075	1.575	0.174	0.132	1.669
150	2pu–1pr	23.651	0.037	3.578	0.223	0.127	2.656
	2pu–1pr Conf	23.412	0.009	2.088	0.201	0.032	2.173
200	2pu–1pr	29.700	0.017	4.800	0.267	0.084	2.782
	2pu–1pr Conf	29.927	0.003	2.770	0.230	0.017	2.534
2pu–1pr; λ_pump, 700 nm; λ_probe, 500 nm; λ_confocal, 450 nm
0	2pu–1pr	0	1	1.610	0.246	1	1.640
	2pu–1pr Conf	0	1	1.269	0.194	1	1.281
50	2pu–1pr	8.240	0.745	1.644	0.247	0.780	1.684
	2pu–1pr Conf	8.240	0.622	1.301	0.195	0.652	1.320
100	2pu–1pr	16.449	0.301	1.794	0.249	0.386	1.893
	2pu–1pr Conf	16.445	0.143	1.476	0.197	0.189	1.531
150	2pu–1pr	24.527	0.064	2.693	0.271	0.161	3.127
	2pu–1pr Conf	24.248	0.012	3.097	0.218	0.043	2.952
200	2pu–1pr	30.398	0.023	3.470	0.316	0.098	3.400
	2pu–1pr Conf	30.586	0.005	2.531	0.247	0.022	2.732
2pu–2pr; λ_pump, 700 nm; λ_probe, 700 nm; λ_confocal, 450 nm
0	2pu–2pr	0	1	1.606	0.246	1	1.612
	2pu–2pr Conf	0	1	1.274	0.195	1	1.276
50	2pu–2pr	8.231	0.743	1.631	0.247	0.760	1.636
	2pu–2pr Conf	8.231	0.620	1.298	0.195	0.643	1.306
100	2pu–2pr	16.455	0.300	1.708	0.248	0.336	1.731
	2pu–2pr Conf	16.446	0.143	1.420	0.197	0.172	1.448
150	2pu–2pr	24.645	0.063	1.948	0.251	0.097	2.054
	2pu–2pr Conf	24.486	0.012	2.067	0.204	0.025	2.086
200	2pu–2pr	29.829	0.007	5.156	0.331	0.036	4.374
	2pu–2pr Conf	30.360	0.002	3.690	0.261	0.008	3.085

Abstract

Cited By

Figures (6)

Tables (1)

Equations (2)

Applied Optics