Abstract

We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.

© 2005 Optical Society of America

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. Gabor, “A new microscopic principle,” Nature 161, 777–778 (1948).
    [CrossRef] [PubMed]
  2. U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002).
    [CrossRef]
  3. I. Yamaguchi, T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22, 1268–1270 (1997).
    [CrossRef] [PubMed]
  4. I. Yamaguchi, J. Kato, S. Ohta, J. Mizuno, “Image formation in phase-shifting digital holography and applications to microscopy,” Appl. Opt. 40, 6177–6185 (2001).
    [CrossRef]
  5. M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
    [CrossRef]
  6. G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
    [CrossRef]
  7. G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
    [CrossRef]
  8. T. Dresel, G. Häusler, H. Venzke, “Three-dimensional sensing of rough surfaces by coherence radar,” Appl. Opt. 31, 919–925 (1992).
    [CrossRef] [PubMed]
  9. B. Nilsson, T. E. Carlsson, “Direct three-dimensional shape measurement by digital-in-flight holography,” Appl. Opt. 37, 7954–7959 (1998).
    [CrossRef]
  10. J. Schmitt, “Optical coherence tomography (OCT): a review,” IEEE J. Sel. Top. Quantum Electron. 5, 1205–1215 (1999).
    [CrossRef]
  11. G. Indebetouw, P. Klysubun, “Optical sectioning with low coherence spatio-temporal holography,” Opt. Commun. 172, 25–29 (1999).
    [CrossRef]
  12. E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
    [CrossRef]
  13. G. Pedrini, H. J. Tiziani, “Short-coherence digital microscopy by use of a lensless holographic imaging system,” Appl. Opt. 41, 4489–4496 (2002).
    [CrossRef] [PubMed]
  14. G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
    [CrossRef]
  15. E. Leith, C. Chen, H. Chen, Y. Chen, D. Dilworth, J. Lopez, J. Rudd, P.-C. Sun, J. Valdmanis, G. Vossler, “Imaging through scattering media with holography,” J. Opt. Soc. Am. A 9, 1148–1153 (1992).
    [CrossRef]

2004 (2)

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

2002 (2)

U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002).
[CrossRef]

G. Pedrini, H. J. Tiziani, “Short-coherence digital microscopy by use of a lensless holographic imaging system,” Appl. Opt. 41, 4489–4496 (2002).
[CrossRef] [PubMed]

2001 (2)

I. Yamaguchi, J. Kato, S. Ohta, J. Mizuno, “Image formation in phase-shifting digital holography and applications to microscopy,” Appl. Opt. 40, 6177–6185 (2001).
[CrossRef]

G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
[CrossRef]

1999 (3)

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

J. Schmitt, “Optical coherence tomography (OCT): a review,” IEEE J. Sel. Top. Quantum Electron. 5, 1205–1215 (1999).
[CrossRef]

G. Indebetouw, P. Klysubun, “Optical sectioning with low coherence spatio-temporal holography,” Opt. Commun. 172, 25–29 (1999).
[CrossRef]

1998 (1)

1997 (2)

I. Yamaguchi, T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22, 1268–1270 (1997).
[CrossRef] [PubMed]

E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
[CrossRef]

1992 (2)

1948 (1)

D. Gabor, “A new microscopic principle,” Nature 161, 777–778 (1948).
[CrossRef] [PubMed]

Bengtsson, B.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Carlsson, T. E.

Chen, C.

Chen, H.

Chen, Y.

Coppola, G.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Cuche, E.

E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
[CrossRef]

De Nicola, S.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Depeursinge, C.

E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
[CrossRef]

Dilworth, D.

Dresel, T.

Egelberg, P.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Ferraro, P.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Finizio, A.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Froning, P.

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

Gabor, D.

D. Gabor, “A new microscopic principle,” Nature 161, 777–778 (1948).
[CrossRef] [PubMed]

Grilli, S.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Gustafsson, M.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Häusler, G.

Indebetouw, G.

G. Indebetouw, P. Klysubun, “Optical sectioning with low coherence spatio-temporal holography,” Opt. Commun. 172, 25–29 (1999).
[CrossRef]

Iodice, M.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

Jüptner, W. P. O.

U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002).
[CrossRef]

Kato, J.

Klysubun, P.

G. Indebetouw, P. Klysubun, “Optical sectioning with low coherence spatio-temporal holography,” Opt. Commun. 172, 25–29 (1999).
[CrossRef]

Leith, E.

Lenart, T.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Lopez, J.

Mendoza-Santoyo, F.

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

Mizuno, J.

Nilsson, B.

Ohta, S.

Pedrini, G.

G. Pedrini, H. J. Tiziani, “Short-coherence digital microscopy by use of a lensless holographic imaging system,” Appl. Opt. 41, 4489–4496 (2002).
[CrossRef] [PubMed]

G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
[CrossRef]

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

Pettersson, S. G.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Poscio, P.

E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
[CrossRef]

Rudd, J.

Schedin, S.

G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
[CrossRef]

Schmitt, J.

J. Schmitt, “Optical coherence tomography (OCT): a review,” IEEE J. Sel. Top. Quantum Electron. 5, 1205–1215 (1999).
[CrossRef]

Schnars, U.

U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002).
[CrossRef]

Sebesta, M.

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Sun, P.-C.

Tiziani, H. J.

G. Pedrini, H. J. Tiziani, “Short-coherence digital microscopy by use of a lensless holographic imaging system,” Appl. Opt. 41, 4489–4496 (2002).
[CrossRef] [PubMed]

G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
[CrossRef]

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

Valdmanis, J.

Venzke, H.

Vossler, G.

Yamaguchi, I.

Zhang, T.

Appl. Opt. (4)

IEEE J. Sel. Top. Quantum Electron. (1)

J. Schmitt, “Optical coherence tomography (OCT): a review,” IEEE J. Sel. Top. Quantum Electron. 5, 1205–1215 (1999).
[CrossRef]

J. Mod. Opt. (1)

G. Pedrini, S. Schedin, H. J. Tiziani, “Aberration compensation in digital holographic reconstruction of microscopic objects,” J. Mod. Opt. 48, 1035–1041 (2001).
[CrossRef]

J. Opt. (1)

E. Cuche, P. Poscio, C. Depeursinge, “Optical tomography by means of a numerical low-coherence holographic technique,” J. Opt. 28, 260–264 (1997).
[CrossRef]

J. Opt. Soc. Am. A (1)

Meas. Sci. Technol. (2)

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15, 529–539 (2004).
[CrossRef]

U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002).
[CrossRef]

Nature (1)

D. Gabor, “A new microscopic principle,” Nature 161, 777–778 (1948).
[CrossRef] [PubMed]

Opt. Commun. (2)

G. Pedrini, P. Froning, H. J. Tiziani, F. Mendoza-Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999).
[CrossRef]

G. Indebetouw, P. Klysubun, “Optical sectioning with low coherence spatio-temporal holography,” Opt. Commun. 172, 25–29 (1999).
[CrossRef]

Opt. Lasers Eng. (1)

M. Gustafsson, M. Sebesta, B. Bengtsson, S. G. Pettersson, P. Egelberg, T. Lenart, “High-resolution digital transmission microscopy: a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004).
[CrossRef]

Opt. Lett. (1)

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Metrics