Abstract

A multiple-trap single-beam scanning laser tweezer system was developed and characterized. Different stationary and mobile multiple-trap modes were generated for polystyrene beads in a water environment. Trapping efficiency and stability were investigated for several dynamic parameters such as transition time between the sites, waiting time on a single site, number of trapping sites, and IR laser power. Optimal parameters for efficient generation of complex arrays and matrices were determined. We demonstrate an example of a single laser beam multiple-trap application by measuring the trap’s stiffness in water for our laser tweezer setup.

© 2005 Optical Society of America

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References

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    [CrossRef]
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2004 (1)

2003 (2)

2002 (3)

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

R. L. Eriksen, P. C. Mogensen, J. Gluckstad, “Multiple-beam optical tweezers generated by the generalized phase-contrast method,” Opt. Lett. 27, 267–269 (2002).
[CrossRef]

2001 (2)

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

M. P. MacDonald, L. Paterson, W. Sibbett, K. Dholakia, P. E. Bryant, “Trapping and manipulation of low-index particles in a two-dimensional interferometric optical trap,” Opt. Lett. 26, 863–865 (2001).
[CrossRef]

2000 (2)

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

1999 (1)

1998 (1)

E. R. Dufresne, D. G. Grier, “Optical tweezers arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1975–1977 (1998).

1991 (1)

Bernet, S.

A. Jesacher, S. Furhapter, S. Bernet, M. Ritsch-Marte, “Diffractive optical tweezers in the Fresnel regime,” Opt. Express 12, 2243–2250 (2004).
[CrossRef] [PubMed]

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

Brouhard, G. J.

G. J. Brouhard, H. T. Schek, A. J. Hunt, “Advanced optical tweezers for the study of cellular and molecular biomechanics,” IEEE Trans. Biomed. Eng. 50, 121–125 (2003).
[CrossRef] [PubMed]

Bryant, P. E.

Cartwright, A. N.

Dearing, M. T.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

Dholakia, K.

Dogterom, M.

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

Dufresne, E. R.

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

E. R. Dufresne, D. G. Grier, “Optical tweezers arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1975–1977 (1998).

Eriksen, R. L.

Faivre-Moskalenko, C.

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

Furhapter, S.

Gluckstad, J.

Gong, T.

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

Greulich, K. O.

K. O. Greulich, Micromanipulation by Light in Biology and Medicine (Bizkhauser-Verlag, 1999).

Grier, D. G.

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

E. R. Dufresne, D. G. Grier, “Optical tweezers arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1975–1977 (1998).

Haist, T.

Hecker, N.

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

Hoogenboom, J. P.

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

Hunt, A. J.

G. J. Brouhard, H. T. Schek, A. J. Hunt, “Advanced optical tweezers for the study of cellular and molecular biomechanics,” IEEE Trans. Biomed. Eng. 50, 121–125 (2003).
[CrossRef] [PubMed]

Jesacher, A.

Kachynski, A. A.

Kaputa, D. S.

Kitamura, N.

Korda, P.

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

Koshioka, M.

Kuzmin, A. N.

MacDonald, M. P.

Marr, D. W. M.

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

Masuhara, H.

Mio, C.

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

Misawa, H.

Mogensen, P. C.

Paterson, L.

Prasad, P. N.

Pudavar, H. E.

Reicherter, M.

Ritsch-Marte, M.

A. Jesacher, S. Furhapter, S. Bernet, M. Ritsch-Marte, “Diffractive optical tweezers in the Fresnel regime,” Opt. Express 12, 2243–2250 (2004).
[CrossRef] [PubMed]

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

Sasaki, K.

Schek, H. T.

G. J. Brouhard, H. T. Schek, A. J. Hunt, “Advanced optical tweezers for the study of cellular and molecular biomechanics,” IEEE Trans. Biomed. Eng. 50, 121–125 (2003).
[CrossRef] [PubMed]

Sheets, S. A.

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

Sibbett, W.

Singer, W.

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

Spalding, G. C.

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

Terray, A.

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

Tiziani, H. J.

van Blaaderenb, A.

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

Vossen, D. L. J.

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

Wagemann, E. U.

Appl. Phys. Lett. (1)

J. P. Hoogenboom, D. L. J. Vossen, C. Faivre-Moskalenko, M. Dogterom, A. van Blaaderenb, “Patterning surfaces with colloidal particles using optical tweezers,” Appl. Phys. Lett. 80, 4828–4830 (2002).
[CrossRef]

IEEE Trans. Biomed. Eng. (1)

G. J. Brouhard, H. T. Schek, A. J. Hunt, “Advanced optical tweezers for the study of cellular and molecular biomechanics,” IEEE Trans. Biomed. Eng. 50, 121–125 (2003).
[CrossRef] [PubMed]

J. Mod. Opt. (1)

W. Singer, S. Bernet, N. Hecker, M. Ritsch-Marte, “Three-dimensional force calibration of optical tweezers,” J. Mod. Opt. 47, 2921–2931 (2000).
[CrossRef]

Opt. Express (1)

Opt. Lett. (5)

Rev. Sci. Instrum. (4)

P. Korda, G. C. Spalding, E. R. Dufresne, D. G. Grier, “Nanofabrication with holographic optical tweezers,” Rev. Sci. Instrum. 73, 1956–1957 (2002).
[CrossRef]

E. R. Dufresne, D. G. Grier, “Optical tweezers arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1975–1977 (1998).

E. R. Dufresne, G. C. Spalding, M. T. Dearing, S. A. Sheets, D. G. Grier, “Computer-generated holographic optical tweezer arrays,” Rev. Sci. Instrum. 72, 1810–1816 (2001).
[CrossRef]

C. Mio, T. Gong, A. Terray, D. W. M. Marr, “Design of a scanning laser optical trap for multiparticle manipulation,” Rev. Sci. Instrum. 71, 2196–2200 (2000).
[CrossRef]

Other (2)

P. N. Prasad, Introduction to Biophotonics (Wiley-Interscience, 2003).
[CrossRef]

K. O. Greulich, Micromanipulation by Light in Biology and Medicine (Bizkhauser-Verlag, 1999).

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Figures (7)

Fig. 1
Fig. 1

The three distinct laser trajectories used for determining the trapping forces: (a) the continuous mode, (b) the interrupted mode, (c) the complex mode.

Fig. 2
Fig. 2

Escaping radius versus the number of points in the circle trajectory for cw and fast transition modes of manipulation.

Fig. 3
Fig. 3

Dependence of the escaping radius on the transition time between two adjacent neighbor trap points of the circle trajectory.

Fig. 4
Fig. 4

Dependence of the escaping radius on the transition time from one outer trajectory site to the next in the complex circle trajectory.

Fig. 5
Fig. 5

Calculated average laser power for complex circle trajectory and measured laser power for the cw mode of bead rotation corresponding to the same escaping radius.

Fig. 6
Fig. 6

Microscope images of multitrap option examples: (a)–(e) Real-time dynamic control of the 3 × 3 matrix of 2.5-µm diameter polystyrene beads in water; tt = 69 µs, tw = 690, P = 196 mW. (f) Stationary trapped 32 beads; tt = tw = 1.15 ms; distance between points, 3.6 µm. (g) Dynamic image (two-photon fluorescence emission under trapping-beam excitation) of four beads in motion (each bead drawing its own character); integration time of the imaging CCD, 2s. Dim portions of the letters p and b are caused by only a single pass of the scanning laser), tt = 460 µs, tw = 1, 15 ms; distance between sites, 1.43 µm.

Fig. 7
Fig. 7

Power dependence of stiffness measured by use of the multitrap single-beam option and the dual-beam option of optical tweezers. Filled circles, multitrap option of one-beam tweezer; open circles, dual-beam tweezers.

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