Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Improvement of transcutaneous fluorescent images with a depth-dependent point-spread function

Not Accessible

Your library or personal account may give you access

Abstract

The point-spread function (PSF) for transcutaneous fluorescent imaging was obtained as an analytical solution in a closed form. It is applicable to cases in which the optical property of the image-blurring turbid medium is considered to be fairly homogeneous. We proposed a technique to improve a transcutaneous image by using depth-dependent PSF. Contrast of the fluorescent image was improved for depths of 1–15 mm in a scattering medium (μs = 1/mm). The visible depth was more than doubled with this technique. An experiment with a rat demonstrated considerable improvement of a transcutaneous image of the cerebral vein at a specified depth. The spread image of the heart was reduced to the correct size by use of the PSF with the actual depth of the heart.

© 2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Three-dimensional transillumination image reconstruction for small animal with new scattering suppression technique

Trung Nghia Tran, Kohei Yamamoto, Takeshi Namita, Yuji Kato, and Koichi Shimizu
Biomed. Opt. Express 5(5) 1321-1335 (2014)

CW transillumination imaging by extracting weakly scattered light from strongly diffused light

Kazuto Takagi, Hiroyuki Kakinuma, Yuji Kato, and Koichi Shimizu
Opt. Express 17(10) 8332-8342 (2009)

Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments

Elie Maalouf, Bruno Colicchio, and Alain Dieterlen
J. Opt. Soc. Am. A 28(9) 1864-1870 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (12)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved