Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light

Abstract

Based on the reflection-type polarization measurement of second-harmonic-generation (SHG) light induced by collagen molecules, we are able to determine the collagen fiber orientation in human tissues taken from a cadaver. The resulting SHG radar graph shows the direction of the absolute orientation and the degree of organization of collagen fibers. To evaluate the probing sensitivity to the collagen orientation, we compared the proposed method with other polarimetric methods. Use of the proposed method revealed characteristic orientation differences among collagen fibers and demonstrated significant inhomogeneity with respect to the distribution of collagen orientation in human dentin. The proposed method provides a powerful research and diagnostic tool for examining the collagen orientation in human tissues.

© 2004 Optical Society of America

Changes of collagen ultrastructure in breast cancer tissue determined by second-harmonic generation double Stokes-Mueller polarimetric microscopy

Ahmad Golaraei, Lukas Kontenis, Richard Cisek, Danielle Tokarz, Susan J. Done, Brian C. Wilson, and Virginijus Barzda
Biomed. Opt. Express 7(10) 4054-4068 (2016)

Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging

Stéphane Bancelin, André Nazac, Bicher Haj Ibrahim, Petr Dokládal, Etienne Decencière, Benjamin Teig, Huda Haddad, Hervé Fernandez, Marie-Claire Schanne-Klein, and Antonello De Martino
Opt. Express 22(19) 22561-22574 (2014)

Correlation between polarization sensitive optical coherence tomography and second harmonic generation microscopy in skin

Viet-Hoan Le, Seunghun Lee, Bumju Kim, Yeoreum Yoon, Calvin J. Yoon, Wan Kyun Chung, and Ki Hean Kim
Biomed. Opt. Express 6(7) 2542-2551 (2015)

Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy

Takeshi Yasui, Yu Takahashi, Shuichiro Fukushima, Yuki Ogura, Toyonobu Yamashita, Tomohiro Kuwahara, Tetsuji Hirao, and Tsutomu Araki
Opt. Express 17(2) 912-923 (2009)

Quantifying collagen structure in breast biopsies using second-harmonic generation imaging

Raghu Ambekar, Tung-Yuen Lau, Michael Walsh, Rohit Bhargava, and Kimani C. Toussaint
Biomed. Opt. Express 3(9) 2021-2035 (2012)