Abstract

The second-harmonic signal in collagen, even in highly organized samples such as rat tail tendon fascicles, varies significantly with position. Previous studies suggest that this variability may be due to the parallel and antiparallel orientation of neighboring collagen fibrils. We applied high-resolution second-harmonic generation microscopy to confirm this hypothesis. Studies in which the focal spot diameter was varied from ∼1 to ∼6 μm strongly suggest that regions in which collagen fibrils have the same orientation in rat tail tendon are likely to be less than ∼1 μm in diameter. These measurements required accurate determination of the focal spot size achieved by use of different microscope objectives; we developed a technique that uses second-harmonic generation in a quartz reference to measure the focal spot diameter directly. We also used the quartz reference to determine a lower limit (d XXX > 0.4 pm/V) for the magnitude of the second-order nonlinear susceptibility in collagen.

© 2003 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
The structural origin of second harmonic generation in fascia

Maxime Rivard, Mathieu Laliberté, Antony Bertrand-Grenier, Catalin Harnagea, Christian P. Pfeffer, Martin Vallières, Yves St-Pierre, Alain Pignolet, My Ali El Khakani, and François Légaré
Biomed. Opt. Express 2(1) 26-36 (2011)

Imaging and modeling collagen architecture from the nano to micro scale

Cameron P. Brown, Marie-Andree Houle, Konstantin Popov, Mischa Nicklaus, Charles-Andre Couture, Matthieu Laliberté, Thomas Brabec, Andreas Ruediger, Andrew J. Carr, Andrew J. Price, Harinderjit S. Gill, Lora Ramunno, and Francois Légaré
Biomed. Opt. Express 5(1) 233-243 (2014)

Collagen and myosin characterization by orientation field second harmonic microscopy

Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Vincent Fleury, and Yann Le Grand
Opt. Express 16(20) 16151-16165 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (4)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (9)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription