Abstract

We have developed a whole-field fluorescence microscope equipped with a Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and the phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration of the fringe period projected onto a sample. We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications.

© 2003 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Phosphorescence lifetime based oxygen micro-sensing using a digital micromirror device

Shih-hui Chao, Mark R. Holl, Sarah C. McQuaide, Timothy T. H. Ren, Steven A. Gales, and Deirdre R. Meldrum
Opt. Express 15(17) 10681-10689 (2007)

Nontranslational three-dimensional profilometry by chromatic confocal microscopy with dynamically configurable micromirror scanning

Sungdo Cha, Paul C. Lin, Lijun Zhu, Pang-Chen Sun, and Yeshaiahu Fainman
Appl. Opt. 39(16) 2605-2613 (2000)

Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy

Zachary J. Smith, Sven Strombom, and Sebastian Wachsmann-Hogiu
Opt. Express 19(18) 16950-16962 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription