Method for evaluating optical characteristics of endoscopes for recording fluorescence-related cardiac electrical activity

Abstract

Nondestructive methods were used to evaluate marketed fiber-optic endoscopes (intended for simple viewing) for fluorescence recording. Our application is for optical recording from the heart. For one angioscope, we measured a focal length of 0.33 mm, a field of view of 45°, an aperture of 0.26 mm, and an efficiency of 43%. We calculated that the angioscope would give a signal-to-noise ratio of 1.0 for a cardiac action potential, if its field of view were divided into a nine-pixel array (for safe continuous illumination). Our methods are useful in designing and evaluating fluorescence fiber-optic systems with superior signal quality and spatial resolution.

© 2002 Optical Society of America

Reconstructing subsurface electrical wave orientation from cardiac epi-fluorescence recordings: Monte Carlo versus diffusion approximation

Christopher J. Hyatt, Christian W. Zemlin, Rebecca M. Smith, Arvydas Matiukas, Arkady M. Pertsov, and Olivier Bernus
Opt. Express 16(18) 13758-13772 (2008)

Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy

Leonardo Sacconi, Jonathan Mapelli, Daniela Gandolfi, Jacopo Lotti, Rodney P. O’Connor, Egidio D’Angelo, and Francesco S. Pavone
Opt. Express 16(19) 14910-14921 (2008)

Miniature endoscope for simultaneous optical coherence tomography and laser-induced fluorescence measurement

Alexandre R. Tumlinson, Lida P. Hariri, Urs Utzinger, and Jennifer K. Barton
Appl. Opt. 43(1) 113-121 (2004)

Depth-resolved optical imaging of transmural electrical propagation in perfused heart

Elizabeth M. C. Hillman, Olivier Bernus, Emily Pease, Matthew B. Bouchard, and Arkady Pertsov
Opt. Express 15(26) 17827-17841 (2007)

Imaging electrical excitation inside the myocardial wall

Bogdan G Mitrea, Bryan J Caldwell, and Arkady M Pertsov
Biomed. Opt. Express 2(3) 620-633 (2011)