Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Determination of surface-bound-fluorophore orientation by goniometric fluorescence polarization: application to quantification of DNA-chip readouts

Not Accessible

Your library or personal account may give you access

Abstract

We present a polarized goniofluorimeter designed to measure the observation-angle and polarization-dependent intensity emitted by a group of surface-bound fluorescent molecules. We studied two types of surface bonding: In one case, dyes were adsorbed into the surface by spin coating, and in the other, dyes were covalently immobilized to DNA strands. Fluorescent dyes consisted of Cy3 and Alexa546. The substrate was a silicon wafer bearing a silicon dioxide layer. The different samples presented a wide panel of reproducible experimental behavior. By confronting experimental behavior with theory and simulation, we can explain these differences as directly linked to the mean orientation of fluorophores with respect to the surface.

© 2002 Optical Society of America

Full Article  |  PDF Article
More Like This
Polarization effects in flow cytometric DNA sizing

Alexandra Agronskaia, Juleon M. Schins, Bart G. de Grooth, and Jan Greve
Appl. Opt. 38(4) 714-719 (1999)

Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements

Adam S. Backer, Maurice Y. Lee, and W. E. Moerner
Optica 3(6) 659-666 (2016)

Backward-enhanced fluorescence from clusters of microspheres and particles of tryptophan

Yong-Le Pan, Steven C. Hill, Jean-Pierre Wolf, Stephen Holler, Richard K. Chang, and Jerold R. Bottiger
Appl. Opt. 41(15) 2994-2999 (2002)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (10)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved