Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Point-spread functions of a polarizing microscope equipped with high-numerical-aperture lenses

Not Accessible

Your library or personal account may give you access

Abstract

In an effort to establish the imaging properties of a new type of polarized-light microscope, we recorded images of small, uniaxial, birefringent crystals. We show that the sequence of in-focus and out-of-focus images, the so-called point-spread function, of a submicroscopic crystal can be used to measure the orientation of its optic axis in three-dimensional space. By analogy to conoscopic images out-of-focus images reveal the changes in relative phase shift between the extraordinary and the ordinary rays that propagate at different directions through the crystal. We also present simulated images of a pointlike anisotropic scattering particle and compare these with our experimental findings. The theoretical model is based on a complete vectorial theory for partial coherent imaging by use of polarized light and high-numerical-aperture lenses.

© 2000 Optical Society of America

Full Article  |  PDF Article
More Like This
Point spread function of the polarized light field microscope

Mai Thi Tran and Rudolf Oldenbourg
J. Opt. Soc. Am. A 39(6) 1095-1103 (2022)

High-numerical-aperture microscopy with a rotating point spread function

Zhixian Yu and Sudhakar Prasad
J. Opt. Soc. Am. A 33(7) B58-B69 (2016)

High focal depth with a quasi-bifocus birefringent lens

Sucharita Sanyal and Ajay Ghosh
Appl. Opt. 39(14) 2321-2325 (2000)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved