Abstract

We investigated the effects of handling and fixation processes on the two-photon fluorescence spectroscopy of endogenous fluorophors in mouse skeletal muscle. The skeletal muscle was handled in one of two ways: either sectioned without storage or sectioned following storage in a freezer. The two-photon fluorescence spectra measured for different storage or fixation periods show a differential among those samples that were stored in water or were fixed either in formalin or methanol. The spectroscopic results indicate that formalin was the least disruptive fixative, having only a weak effect on the two-photon fluorescence spectroscopy of muscle tissue, whereas methanol had a significant influence on one of the autofluorescence peaks. The two handling processes yielded similar spectral information, indicating no different effects between them.

© 2000 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Interrogation of metabolic and oxygen states of tumors with fiber-based luminescence lifetime spectroscopy

Maria Lukina, Anna Orlova, Marina Shirmanova, Daniil Shirokov, Anton Pavlikov, Antje Neubauer, Hauke Studier, Wolfgang Becker, Elena Zagaynova, Toshitada Yoshihara, Seiji Tobita, and Vladislav Shcheslavskiy
Opt. Lett. 42(4) 731-734 (2017)

Fluorescence time-resolved macroimaging

Vladislav I. Shcheslavskiy, Marina V. Shirmanova, Varvara V. Dudenkova, Konstantin A. Lukyanov, Alena I. Gavrina, Anastasia V. Shumilova, Elena Zagaynova, and Wolfgang Becker
Opt. Lett. 43(13) 3152-3155 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription