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Hybrid holographic microscopy: visualization of three-dimensional object information by use of viewing angles

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Abstract

One of the attractive features of hybrid holographic microscopy, in which the hologram of a microscopic object recorded by an image sensor is numerically reconstructed with a computer, is that the three-dimensional (3-D) information of a recorded object is obtained. The 3-D information has often been extracted by means of changing the reconstruction distance in the numerical reconstruction process, but here we describe an alternative technique that allows for variable viewing angles. That is, the perspective from which the object is viewed can be varied. The approximation used enables use of the fast-Fourier-transform algorithm for numerical reconstruction even in the high-resolution case in which the Fresnel approximation is no longer valid. The resolution of the proposed technique is also discussed.

© 2000 Optical Society of America

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