Abstract

An infrared spectral analysis of human blood serum has been carried out with Fourier transform infrared fiber-optic evanescent-wave spectroscopy. The measured spectra were analyzed by neural network analysis to predict concentrations of cholesterol, creatinine, total protein, urea, and uric acid. The predicted concentrations were compared with results from standard chemical analysis of blood and a good correlation was observed.

© 2000 Optical Society of America

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References

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  1. R. J. Henry, D. C. Cannon, J. W. Winkelman, Clinical Chemistry, 2nd ed. (Harper and Row, New York, 1974).
  2. H. H. Mantch, D. Chapman, Infrared Spectroscopy of Biomolecules (Wiley-Liss, New York, 1996).
  3. R. Simhi, Y. Gotshal, D. Bunimovich, B.-A. Sela, A. Katzir, “Fiber-optic evanescent-wave spectroscopy for fast multicomponent analysis of human blood,” Appl. Opt. 35, 3421–3425 (1996).
    [CrossRef] [PubMed]
  4. Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
    [CrossRef]
  5. E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.
  6. P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
    [CrossRef]
  7. H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
    [CrossRef] [PubMed]
  8. G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
    [CrossRef]
  9. K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
    [CrossRef]
  10. H. M. Heise, B. Bittner, “Investigation of experimental error in the quantitative analysis of glucose in human blood plasma by ATR-IR spectroscopy,” J. Mol. Struct. 348, 21–24 (1995).
    [CrossRef]
  11. H. M. Heise, R. Marbach, T. H. Koschinski, F. A. Gries, “Multicomponent assay for blood substrates in human plasma by mid-infrared spectroscopy and its evaluation for clinical analysis,” Appl. Spectrosc. 48, 85–95 (1994).
    [CrossRef]

1997 (1)

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

1996 (1)

1995 (1)

H. M. Heise, B. Bittner, “Investigation of experimental error in the quantitative analysis of glucose in human blood plasma by ATR-IR spectroscopy,” J. Mol. Struct. 348, 21–24 (1995).
[CrossRef]

1994 (2)

H. M. Heise, R. Marbach, T. H. Koschinski, F. A. Gries, “Multicomponent assay for blood substrates in human plasma by mid-infrared spectroscopy and its evaluation for clinical analysis,” Appl. Spectrosc. 48, 85–95 (1994).
[CrossRef]

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

1993 (1)

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

1989 (2)

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

Bhandare, P.

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.

Bittner, B.

H. M. Heise, B. Bittner, “Investigation of experimental error in the quantitative analysis of glucose in human blood plasma by ATR-IR spectroscopy,” J. Mol. Struct. 348, 21–24 (1995).
[CrossRef]

Bunimovich, D.

Cannon, D. C.

R. J. Henry, D. C. Cannon, J. W. Winkelman, Clinical Chemistry, 2nd ed. (Harper and Row, New York, 1974).

Chapman, D.

H. H. Mantch, D. Chapman, Infrared Spectroscopy of Biomolecules (Wiley-Liss, New York, 1996).

Gotshal, Y.

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

R. Simhi, Y. Gotshal, D. Bunimovich, B.-A. Sela, A. Katzir, “Fiber-optic evanescent-wave spectroscopy for fast multicomponent analysis of human blood,” Appl. Opt. 35, 3421–3425 (1996).
[CrossRef] [PubMed]

Gries, F. A.

Hasiguchi, Y.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Heise, H. M.

H. M. Heise, B. Bittner, “Investigation of experimental error in the quantitative analysis of glucose in human blood plasma by ATR-IR spectroscopy,” J. Mol. Struct. 348, 21–24 (1995).
[CrossRef]

H. M. Heise, R. Marbach, T. H. Koschinski, F. A. Gries, “Multicomponent assay for blood substrates in human plasma by mid-infrared spectroscopy and its evaluation for clinical analysis,” Appl. Spectrosc. 48, 85–95 (1994).
[CrossRef]

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

Henry, R. J.

R. J. Henry, D. C. Cannon, J. W. Winkelman, Clinical Chemistry, 2nd ed. (Harper and Row, New York, 1974).

Ichinose, K.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Janatsch, G.

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

Katzir, A.

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

R. Simhi, Y. Gotshal, D. Bunimovich, B.-A. Sela, A. Katzir, “Fiber-optic evanescent-wave spectroscopy for fast multicomponent analysis of human blood,” Appl. Opt. 35, 3421–3425 (1996).
[CrossRef] [PubMed]

Kishikawa, H.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Kjiwara, K.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Koschinski, T. H.

Kruse-Jarres, J. D.

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

Mantch, H. H.

H. H. Mantch, D. Chapman, Infrared Spectroscopy of Biomolecules (Wiley-Liss, New York, 1996).

Marbach, R.

H. M. Heise, R. Marbach, T. H. Koschinski, F. A. Gries, “Multicomponent assay for blood substrates in human plasma by mid-infrared spectroscopy and its evaluation for clinical analysis,” Appl. Spectrosc. 48, 85–95 (1994).
[CrossRef]

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

Meldenson, Y.

E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.

Mendelson, Y.

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

Nishida, K.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Peura, R. A.

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.

Sakakida, M.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Sela, B.

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

Sela, B.-A.

Shichiri, M.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Simhi, R.

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

R. Simhi, Y. Gotshal, D. Bunimovich, B.-A. Sela, A. Katzir, “Fiber-optic evanescent-wave spectroscopy for fast multicomponent analysis of human blood,” Appl. Opt. 35, 3421–3425 (1996).
[CrossRef] [PubMed]

Stohr, E.

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.

Uehara, M.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Uemura, T.

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Winkelman, J. W.

R. J. Henry, D. C. Cannon, J. W. Winkelman, Clinical Chemistry, 2nd ed. (Harper and Row, New York, 1974).

Anal. Chem. (2)

H. M. Heise, R. Marbach, G. Janatsch, J. D. Kruse-Jarres, “Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef] [PubMed]

G. Janatsch, J. D. Kruse-Jarres, R. Marbach, H. M. Heise, “Multivariate calibration for assay in clinical chemistry using attenuated total reflection infrared spectra of human blood plasma,” Anal. Chem. 61, 2009–2015 (1989).
[CrossRef]

Appl. Opt. (1)

Appl. Spectrosc. (1)

J. Mol. Struct. (1)

H. M. Heise, B. Bittner, “Investigation of experimental error in the quantitative analysis of glucose in human blood plasma by ATR-IR spectroscopy,” J. Mol. Struct. 348, 21–24 (1995).
[CrossRef]

Med. Biol. Eng. Comput. (1)

K. Kjiwara, T. Uemura, H. Kishikawa, K. Nishida, Y. Hasiguchi, M. Uehara, M. Sakakida, K. Ichinose, M. Shichiri, “Noninvasive measurements of blood glucose concentrations by analyzing Fourier transform infrared absorbance spectra through oral mucosa,” Med. Biol. Eng. Comput. 31, S17–S22 (1993).
[CrossRef]

Sens. Actuators B (1)

Y. Gotshal, R. Simhi, B. Sela, A. Katzir, “Blood diagnostics using fiber-optic evanescent wave spectroscopy and neural networks analysis,” Sens. Actuators B 42, 157–161 (1997).
[CrossRef]

Vib. Spectrosc. (1)

P. Bhandare, Y. Mendelson, E. Stohr, R. A. Peura, “Glucose determination in simulated serum solutions by Fourier transform infrared spectroscopy: investigation of spectral interferences,” Vib. Spectrosc. 6, 363–378 (1994).
[CrossRef]

Other (3)

E. Stohr, P. Bhandare, R. A. Peura, Y. Meldenson, “Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in vitro measurement of blood glucose,” in Proceedings of the Eighteenth IEEE Annual Northeast Bioengineering Conference (Institute of Electrical and Electronic Engineers, New York, 1992), pp. 105–106.

R. J. Henry, D. C. Cannon, J. W. Winkelman, Clinical Chemistry, 2nd ed. (Harper and Row, New York, 1974).

H. H. Mantch, D. Chapman, Infrared Spectroscopy of Biomolecules (Wiley-Liss, New York, 1996).

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Figures (3)

Fig. 1
Fig. 1

(a) Experimental setup; (b) details of a segment of the sensing element.

Fig. 2
Fig. 2

Dependence of MSE on the number of iterations.

Fig. 3
Fig. 3

Absorbance at 1600 cm-1 of various concentrations of urea in a 0.9% saline solution.

Tables (3)

Tables Icon

Table 1 Concentration of Blood Constituents as Obtained from Chemical Analysisa

Tables Icon

Table 2 Concentrations of Blood Constituents by Leave-One-Out Methods for 60 Samples Originally Used in the Training Step

Tables Icon

Table 3 Predicted Concentrations of Completely Unknown Samples (20 Samples)a

Metrics