Spatial distribution of single-photon and two-photon fluorescence light in scattering media: Monte Carlo simulation

Abstract

Three-dimensional fluorescence spatial distributions under single-photon and two-photon excitation within a turbid medium are studied with Monte Carlo simulation. It is demonstrated that two-photon excitation has an advantage of producing much less fluorescence light outside the focal region compared with single-photon excitation. With the increase of the concentration of scattering particles in a turbid medium, the position of the maximum fluorescence intensity point shifts from the geometric focal region toward the medium surface. Further studies show that the optical sectioning property of two-photon fluorescence microscopy is degraded in thick turbid media or when the numerical aperture of an objective becomes low.

© 2000 Optical Society of America

Discussion of methods for depth enhancement in single and multiphoton-stimulated emission microscopy

Robert D. Frankel
J. Opt. Soc. Am. B 33(7) 1421-1438 (2016)

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization

Chad E. Bigelow and Thomas H. Foster
J. Opt. Soc. Am. A 23(11) 2932-2943 (2006)

Simulation of confocal microscopy through scattering media with and without time gating

Marcus Magnor, Peter Dorn, and Wolfgang Rudolph
J. Opt. Soc. Am. B 18(11) 1695-1700 (2001)

Effective point-spread function for fast image modeling and processing in microscopic imaging through turbid media

Xiaosong Gan and Min Gu
Opt. Lett. 24(11) 741-743 (1999)

Excitation with a focused, pulsed optical beam in scattering media: diffraction effects

Vincent Ricardo Daria, Caesar Saloma, and Satoshi Kawata
Appl. Opt. 39(28) 5244-5255 (2000)