Abstract
Three-dimensional fluorescence spatial distributions under
single-photon and two-photon excitation within a turbid medium are
studied with Monte Carlo simulation. It is demonstrated that
two-photon excitation has an advantage of producing much less
fluorescence light outside the focal region compared with single-photon
excitation. With the increase of the concentration of scattering
particles in a turbid medium, the position of the maximum fluorescence
intensity point shifts from the geometric focal region toward the
medium surface. Further studies show that the optical sectioning
property of two-photon fluorescence microscopy is degraded in thick
turbid media or when the numerical aperture of an objective becomes
low.
© 2000 Optical Society of America
Full Article |
PDF Article
OSA Recommended Articles
References
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access OSA Member Subscription
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access OSA Member Subscription
Tables (1)
You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access OSA Member Subscription
Equations (3)
You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access OSA Member Subscription
Metrics
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access OSA Member Subscription