Abstract

We describe a nitrogen dye laser—optical multichannel analyzer (OMA) system capable of quickly measuring tissue autofluorescence spectra in vivo at multiple excitation wavelengths. Sample spectra were acquired successfully from patients undergoing gastrointestinal (GI) endoscopic examination.

© 1999 Optical Society of America

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References

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  1. R. Richards-Kortum et al., “Spectroscopic diagnosis of colonic dysplasia,” Photochem. Photobiol. 53,777–786 (1991).
    [PubMed]
  2. A. Mahadevan et al., “Study of the fluorescence properties of normal and neoplastic human cervical tissue,” Lasers Surg. Med. 13,647–655 (1993).
    [CrossRef] [PubMed]
  3. H. Zeng et al., “Spectroscopic and microscopic characteristics of human skin autofluorescence emission,” Photochem. Photobiol. 61,639–645 (1995).
    [CrossRef] [PubMed]
  4. D.R. Ingrams et al., “Autofluorescence characteristics of oral mucosa,” Head & Neck 19,27–32 (1997).
    [CrossRef]
  5. R.A. Zângaro et al., “Rapid multi-excitation fluorescence spectroscopy system for in vivo tissue diagnosis,” Appl. Opt. 35,5211–5219 (1996).
    [CrossRef]

1997 (1)

D.R. Ingrams et al., “Autofluorescence characteristics of oral mucosa,” Head & Neck 19,27–32 (1997).
[CrossRef]

1996 (1)

1995 (1)

H. Zeng et al., “Spectroscopic and microscopic characteristics of human skin autofluorescence emission,” Photochem. Photobiol. 61,639–645 (1995).
[CrossRef] [PubMed]

1993 (1)

A. Mahadevan et al., “Study of the fluorescence properties of normal and neoplastic human cervical tissue,” Lasers Surg. Med. 13,647–655 (1993).
[CrossRef] [PubMed]

1991 (1)

R. Richards-Kortum et al., “Spectroscopic diagnosis of colonic dysplasia,” Photochem. Photobiol. 53,777–786 (1991).
[PubMed]

Ingrams, D.R.

D.R. Ingrams et al., “Autofluorescence characteristics of oral mucosa,” Head & Neck 19,27–32 (1997).
[CrossRef]

Mahadevan, A.

A. Mahadevan et al., “Study of the fluorescence properties of normal and neoplastic human cervical tissue,” Lasers Surg. Med. 13,647–655 (1993).
[CrossRef] [PubMed]

Richards-Kortum, R.

R. Richards-Kortum et al., “Spectroscopic diagnosis of colonic dysplasia,” Photochem. Photobiol. 53,777–786 (1991).
[PubMed]

Zângaro, R.A.

Zeng, H.

H. Zeng et al., “Spectroscopic and microscopic characteristics of human skin autofluorescence emission,” Photochem. Photobiol. 61,639–645 (1995).
[CrossRef] [PubMed]

Appl. Opt. (1)

Head & Neck (1)

D.R. Ingrams et al., “Autofluorescence characteristics of oral mucosa,” Head & Neck 19,27–32 (1997).
[CrossRef]

Lasers Surg. Med. (1)

A. Mahadevan et al., “Study of the fluorescence properties of normal and neoplastic human cervical tissue,” Lasers Surg. Med. 13,647–655 (1993).
[CrossRef] [PubMed]

Photochem. Photobiol. (2)

H. Zeng et al., “Spectroscopic and microscopic characteristics of human skin autofluorescence emission,” Photochem. Photobiol. 61,639–645 (1995).
[CrossRef] [PubMed]

R. Richards-Kortum et al., “Spectroscopic diagnosis of colonic dysplasia,” Photochem. Photobiol. 53,777–786 (1991).
[PubMed]

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Figures (3)

Figure 1
Figure 1

(a) Block diagram and (b) timing series of operation of the system.

Figure 2
Figure 2

Fluorescence spectra of a phantom (Coumarin 540 and Rhodamine 590 mixed with methanol) at different excitation wavelengths.

Figure 3
Figure 3

(a) Fluorescence spectra of a normal colonic tissue and (b) of a tubular adenoma at five different excitation wavelengths.

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