Abstract
We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.
© 1999 Optical Society of America
Full Article | PDF ArticleOSA Recommended Articles
Tristan Colomb, Etienne Cuche, Florian Charrière, Jonas Kühn, Nicolas Aspert, Frédéric Montfort, Pierre Marquet, and Christian Depeursinge
Appl. Opt. 45(5) 851-863 (2006)
Lluís Martínez-León, Giancarlo Pedrini, and Wolfgang Osten
Appl. Opt. 44(19) 3977-3984 (2005)
Etienne Cuche, Frédéric Bevilacqua, and Christian Depeursinge
Opt. Lett. 24(5) 291-293 (1999)