Abstract

It is known that signal level in single-, two- and three-photon confocal fluorescence microscopy increases with the size of the detector. Here we evaluate the signal-to-noise and the signal-to-background criteria for these microscopes. We investigate the effect of pinhole size on their ability to detect a weakly fluorescent point object in the presence of a uniformly fluorescence background. Numerical results based on a paraxial approximation theory show that optimization of these criteria gives an optimal value for pinhole size, which results in an improved imaging performance. The resulting improvement in noise performance, compared with the use of a large detector, is greater for three-photon than for two-photon confocal fluorescence microscopes.

© 1999 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Interpretation of the optical transfer function: Significance for image scanning microscopy

Colin J. R. Sheppard, Stephan Roth, Rainer Heintzmann, Marco Castello, Giuseppe Vicidomini, Rui Chen, Xudong Chen, and Alberto Diaspro
Opt. Express 24(24) 27280-27287 (2016)

Improved spatial resolution in fluorescence focal modulation microscopy

Wei Gong, Ke Si, Nanguang Chen, and Colin J. R. Sheppard
Opt. Lett. 34(22) 3508-3510 (2009)

Quantitative comparison of background rejection, signal-to-noise ratio, and resolution in confocal and full-field laser scanning microscopes

D. R. Sandison, D. W. Piston, R. M. Williams, and W. W. Webb
Appl. Opt. 34(19) 3576-3588 (1995)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (11)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription