Abstract

Laser sources offer a potentially low-cost means of improving the light throughput in tandem-scanning confocal microscopy because of their high beam directionality. We measure and compare the optical sectioning characteristics of the tandem-scanning microscope (TSM) employing (i) the traditional choice of incoherent light from a Xe arc lamp and (ii) a cited alternative—coherent light from a He–Ne laser source. In general the laser source is found to result in axial responses with pronounced sidelobes, the sizes and locations of which are extremely sensitive to the alignment of the pinhole array. The implications of these results for practical TSM systems are discussed.

© 1998 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Effects of source coherence and aperture array geometry on optical sectioning strength in direct-view microscopy

Cian M. Taylor and Eithne M. McCabe
J. Opt. Soc. Am. A 19(7) 1406-1416 (2002)

Confocal microscopy using variable-focal-length microlenses and an optical fiber bundle

Lisong Yang, Aaron Mac Raighne, Eithne M. McCabe, L. Andrea Dunbar, and Toralf Scharf
Appl. Opt. 44(28) 5928-5936 (2005)

Influence of source coherence and aperture distribution on the imaging properties in direct-view microscopy

S. J. Hewlett, D. T. Fewer, and E. M. McCabe
J. Opt. Soc. Am. A 14(5) 1066-1075 (1997)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription