Abstract
Laser sources offer a potentially low-cost means of improving the
light throughput in tandem-scanning confocal microscopy because of
their high beam directionality. We measure and compare the optical
sectioning characteristics of the tandem-scanning microscope (TSM)
employing (i) the traditional choice of incoherent light from a Xe
arc lamp and (ii) a cited alternative—coherent light from a He–Ne
laser source. In general the laser source is found to result in
axial responses with pronounced sidelobes, the sizes and locations of
which are extremely sensitive to the alignment of the pinhole
array. The implications of these results for practical TSM systems
are discussed.
© 1998 Optical Society of America
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