Abstract

We describe a near-field scanning optical microscope capable of imaging biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical shear-force feedback for tip–sample distance regulation. Physical aspects of the design are discussed, and phenomena related to operation in liquid are revealed. Careful calibration of the instrument in air and in liquid is shown, and for the first time to our knowledge, near-field fluorescence images of a biological cell in liquid are presented.

© 1998 Optical Society of America

Full Article  |  PDF Article
Related Articles
Near-field optical microscope based on local perturbation of a diffraction spot

R. Bachelot, P. Gleyzes, and A. C. Boccara
Opt. Lett. 20(18) 1924-1926 (1995)

Method for increasing shear-force detection sensitivity with uncoated fiber tips

C. Durkan and I. V. Shvets
Appl. Opt. 36(31) 8173-8178 (1997)

Correlation between optical and topographical images from an external reflection near-field microscope with shear force feedback

Sergey I. Bozhevolnyi, Igor I. Smolyaninov, and Ole Keller
Appl. Opt. 34(19) 3793-3799 (1995)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription