Abstract

We present experiments to predict the maximum penetration depth at which typical biological structures in amelanotic tissue can be detected with confocal microscopy. The detected signal is examined as the signal source strength (index of refraction mismatch), the source depth, and the medium scattering coefficient are varied. The detected background produced by scattering outside the focal volume is examined as the medium scattering coefficient, the depth in the medium, the dimensionless pinhole radius, ν p, and the shape of the scattering phase function are varied. When the system approaches ideal confocal performance (ν p ≃ 3), the penetration depth is limited by the signal-to-noise ratio to approximately 3–4 optical depths (OD’s) for a 0.05 index mismatch. As ν p increases to 8, the penetration depth is limited by the signal-to-background ratio and is dependent on the scattering coefficient. At μs= 100 cm-1 (l s = 100 μm) and an index mismatch of 0.05, the maximum penetration depth is approximately 2 OD.

© 1998 Optical Society of America

Full Article  |  PDF Article

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (5)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription