We describe the quantitative analysis of some metabolic gases by multichannel Raman spectroscopy. Raman spectra were measured for air, acetone, ammonia, carbon dioxide, and mixed gas consisting of acetone, ammonia, and air. We designed a new elliptic–spherical integration type of cell holder to obtain the Raman spectra of gases with a high signal-to-noise ratio. Concentrations of acetone, ammonia, and carbon dioxide were determined by the peak intensities of Raman bands at 2940, 3228, and 1385 cm-1, respectively. To compensate for the fluctuations of Raman intensities caused by several factors, such as the fluctuations of laser power, the peak intensity of a band at 2324 cm-1 due to nitrogen gas was used as an internal intensity standard. The correlation coefficient between the corrected Raman intensity at 2940 cm-1 and the concentration of acetone was calculated to be 0.984 for a concentration range of 2–12 ppm. The detection limit of acetone gas was found to be 2 ppm.
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