Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Two-dimensional visualization of fluorescence lifetimes by use of a picosecond laser and a streak camera

Not Accessible

Your library or personal account may give you access

Abstract

Two-dimensional distributions of the effective lifetime of the fluorescence emission induced by short-pulsed laser radiation are obtained from two-dimensional images recorded with a streak camera and a charge-coupled device by means of a separation algorithm method (SAM). In theory, the best response with respect to noise is obtained for lifetimes corresponding to a range of pixels of 5–50 in the CCD, that is, 5–50 ps at the fastest streak speed. In experiments the SAM is compared with pure time-resolved measurements, and it is used for two-dimensional lifetime evaluation. The laser-pulse duration is 25 ps, and the lower limit of the lifetime resolution as used in the experiments is estimated to be 200–250 ps. The results demonstrate the possibility of performing pattern recognition independently of the relative distribution of emission intensity between regions of different fluorescence lifetimes. The technique is demonstrated for static objects but can in principle be extended to nonstationary objects if two detectors are used.

© 1998 Optical Society of America

Full Article  |  PDF Article
More Like This
Three-dimensional flow visualization with picosecond Mie scattering and streak-camera detection

Frederik Ossler, Sara Agrup, and Marcus Aldén
Appl. Opt. 34(3) 537-540 (1995)

Fluorescence lifetime imaging with picosecond resolution for biomedical applications

K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw
Opt. Lett. 23(10) 810-812 (1998)

Picosecond planar laser-induced fluorescence measurements of OH A2 Σ+ (ν′ = 2) lifetime and energy transfer in atmospheric pressure flames

Frank C. Bormann, Tim Nielsen, Michael Burrows, and Peter Andresen
Appl. Opt. 36(24) 6129-6140 (1997)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (12)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved