Abstract

A fluorescence imaging device applied to the detection of early cancer is described. The apparatus is based on the imaging of laser-induced fluorescence of a dye that localizes in a tumor with a higher concentration than in the surrounding normal tissue after iv injection. Tests carried out in the upper aerodigestive tract, the tracheobronchial tree, and the esophagus with Photofrin II (1 mg/kg of body weight) as the fluorescent agent are reported as examples. The fluorescence is induced by violet (410-nm) light from a continuous-wave (cw) krypton-ion laser. The fluorescence contrast between tumor and surrounding tissue is enhanced by real-time image processing. This is done by the simultaneous recording of the fluorescence image in two spectral domains (470–600 and 600–720 nm), after which these two images are digitized and manipulated with a mathematical operator (look-up table) at video frequency. Among the 7 photodetections performed in the tracheobronchial tree, 6 were successful, whereas it was the case for only 5 of the 15 lesions investigated in squamous mucosa (upper aerodigestive tract and esophagus). The sources of false positives and false negatives are evaluated in terms of the fluorescent dye, tissue optical properties, and illumination optics.

© 1997 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Fluorescence-guided optical coherence tomography imaging for colon cancer screening: a preliminary mouse study

Nicusor Iftimia, Arun K. Iyer, Daniel X. Hammer, Niyom Lue, Mircea Mujat, Martha Pitman, R. Daniel Ferguson, and Mansoor Amiji
Biomed. Opt. Express 3(1) 178-191 (2012)

Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy

Gyungseok Oh, Su Woong Yoo, Yebin Jung, Yeon-Mi Ryu, Youngrong Park, Sang-Yeob Kim, Ki Hean Kim, Sungjee Kim, Seung-Jae Myung, and Euiheon Chung
Biomed. Opt. Express 5(5) 1677-1689 (2014)

Wide-field fluorescence lifetime imaging of cancer

James McGinty, Neil P. Galletly, Chris Dunsby, Ian Munro, Daniel S. Elson, Jose Requejo-Isidro, Patrizia Cohen, Raida Ahmad, Amanda Forsyth, Andrew V. Thillainayagam, Mark A. A. Neil, Paul M. W. French, and Gordon W Stamp
Biomed. Opt. Express 1(2) 627-640 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription