Abstract

A new method is proposed for analyzing a transient curve as a multiexponential decay convolved with an instrumental response function and for estimating the decay constants and amplitudes without knowledge of the response function. The method requires a single-exponential reference curve observed with the same response function as that for a sample curve, whereas its decay-constant and amplitude values are not necessary for the estimation. The sample and reference curves are analyzed with a convolved autoregressive model, which is based on a linear least-squares algorithm. This method has the advantage of a short computation time when compared with a conventional nonlinear least-squares fitting technique. Double-exponential curves are also acceptable as the reference, if two curves with different decay parameters can be provided for the analysis.

© 1996 Optical Society of America

Full Article  |  PDF Article
More Like This
Analysis of transient emission curves by a convolved autoregressive model

Keiji Sasaki and Hiroshi Masuhara
Appl. Opt. 30(8) 977-980 (1991)

Quantitative analysis of decay transients applied to a multimode pulsed cavity ringdown experiment

Hans Naus, Ivo H. M. van Stokkum, Wim Hogervorst, and Wim Ubachs
Appl. Opt. 40(24) 4416-4426 (2001)

Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry

Chiranjit Deka and John A. Steinkamp
Appl. Opt. 35(22) 4481-4489 (1996)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (1)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (23)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription