Abstract

A simple method based on total internal reflection is presented for measuring the refractive index of biological tissue. A narrow laser beam and a semicylindrical lens in contact with a tissue specimen are used in the experimental apparatus. The dependence of the internal reflectivity on the angle of incidence is measured to determine the critical angle and therefore the refractive index of tissue. The experimental results for several tissue samples show that the method is reliable and useful for studying tissue optics. The principal advantages of the method are its elimination of scattering effects, suitability to a small in vitro sample, and excellent accuracy.

© 1996 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Experimental measurement of the refractive index of biological tissues by total internal reflection

Jiancheng Lai, Zhenhua Li, Chunyong Wang, and Anzhi He
Appl. Opt. 44(10) 1845-1849 (2005)

Determination of refractive indices of porcine skin tissues and Intralipid at eight wavelengths between 325 and 1557 nm

Huafeng Ding, Jun Q. Lu, Kenneth M. Jacobs, and Xin-Hua Hu
J. Opt. Soc. Am. A 22(6) 1151-1157 (2005)

Selective delivery of laser energy to biological tissue based on refractive-index differences

Yacov Domankevitz, Jerry Waldman, Charles P. Lin, and R. Rox Anderson
Opt. Lett. 25(15) 1104-1106 (2000)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription