Abstract

We present a single-ended technique for three-dimensional imaging of objects embedded in a turbid medium by the use of time-resolved fluorescence emission or Raman scattering. The technique uses the earliest arriving photons, which we show are not sensitive to the relatively long fluorescence lifetime, and thus can be used to extract the desired spatial information accurately, even at a distance equivalent to 100 mean free paths. The results also demonstrate the feasibility and the potential of one’s combining time-resolved optical tomography with fluorescence or Raman spectroscopy to localize and identify the embedded objects. This technique may be valuable for the diagnosis of disease in highly scattering human tissue because it can provide spatial and biochemical information about the composition of embedded lesions.

© 1995 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Time-resolved multichannel imaging of fluorescent objects embedded in turbid media

Jun Wu, Yang Wang, Lev Perelman, Irving Itzkan, Ramachandra R. Dasari, and Michael S. Feld
Opt. Lett. 20(5) 489-491 (1995)

Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates

Jin Chen, Vivek Venugopal, and Xavier Intes
Biomed. Opt. Express 2(4) 871-886 (2011)

Time-gated transillumination of biological tissues and tissuelike phantoms

Gerhard Mitic, Jochen Kölzer, Johann Otto, Erich Plies, Gerald Sölkner, and Wolfgang Zinth
Appl. Opt. 33(28) 6699-6710 (1994)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription