Abstract

Dynamic biospeckle can be obtained from cultures of motile microorganisms by use of a hybrid opto-electronic system, and it carries feature information about the cultures. On the basis of theoretical analysis and experimental results we describe and discuss two techniques that process the signals derived from dynamic biospeckle. Experimental results on detection of space–time autocorrelation and analysis of histograms of subtraction between two speckle patterns are demonstrated. After extracting dynamic and statistical characteristics of the cultures of motile microorganisms, we show that with these techniques, real-time and in situ culture vitality monitoring and automatic culture identifications are possible.

© 1994 Optical Society of America

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