Abstract

We demonstrate the feasibility of using a pulsed copper vapor laser in particle detection and sizing Despite the low-duty cycle of these lasers (0.025%), the forward scatter of light from 5-μm latex spheres has been detected.

© 1993 Optical Society of America

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References

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  1. M. Kerker, The Scattering of Light and Other Electromagnetic Radiation (Academic, New York, 1969), Chap. 7, p. 311.
  2. C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, Amsterdam, 1983), Chap. 13, p. 381.
  3. J. A. Steinkamp, “Flow cytometry,” Rev. Sci. Instrum. 55, 1375–1400(1984).
    [CrossRef]
  4. M. R. Melamed, P. F. Mullaney, M. L. Mendelsohn, eds., Flow Cytometry and Sorting (Wiley, New York, 1979), Chap. 21, p. 383.
  5. P. F. Mullaney, P. N. Dean, “Cell sizing: a small-angle light-scattering method for sizing particles of low refractive index,” Appl. Opt. 8, 2361–2362 (1969).
    [CrossRef]
  6. P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
    [CrossRef] [PubMed]
  7. L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
    [CrossRef] [PubMed]

1984 (1)

J. A. Steinkamp, “Flow cytometry,” Rev. Sci. Instrum. 55, 1375–1400(1984).
[CrossRef]

1976 (1)

L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
[CrossRef] [PubMed]

1969 (2)

P. F. Mullaney, P. N. Dean, “Cell sizing: a small-angle light-scattering method for sizing particles of low refractive index,” Appl. Opt. 8, 2361–2362 (1969).
[CrossRef]

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

Bohren, C. F.

C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, Amsterdam, 1983), Chap. 13, p. 381.

Coulter, J. R.

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

Dean, P. N.

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

P. F. Mullaney, P. N. Dean, “Cell sizing: a small-angle light-scattering method for sizing particles of low refractive index,” Appl. Opt. 8, 2361–2362 (1969).
[CrossRef]

Herzenberg, L. A.

L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
[CrossRef] [PubMed]

L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
[CrossRef] [PubMed]

Huffman, D. R.

C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, Amsterdam, 1983), Chap. 13, p. 381.

Kerker, M.

M. Kerker, The Scattering of Light and Other Electromagnetic Radiation (Academic, New York, 1969), Chap. 7, p. 311.

Mullaney, P. F.

P. F. Mullaney, P. N. Dean, “Cell sizing: a small-angle light-scattering method for sizing particles of low refractive index,” Appl. Opt. 8, 2361–2362 (1969).
[CrossRef]

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

Steinkamp, J. A.

J. A. Steinkamp, “Flow cytometry,” Rev. Sci. Instrum. 55, 1375–1400(1984).
[CrossRef]

Sweet, R. G.

L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
[CrossRef] [PubMed]

Van Dilla, M. A.

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

Appl. Opt. (1)

Rev. Sci. Instrum. (2)

P. F. Mullaney, M. A. Van Dilla, J. R. Coulter, P. N. Dean, “Cell sizing: a light scattering photometer for rapid volume determination,” Rev. Sci. Instrum. 40, 1029–1032 (1969).
[CrossRef] [PubMed]

J. A. Steinkamp, “Flow cytometry,” Rev. Sci. Instrum. 55, 1375–1400(1984).
[CrossRef]

Sci. Am. (1)

L. A. Herzenberg, R. G. Sweet, L. A. Herzenberg, “Fluorescence-activated cell sorting,” Sci. Am. 234, 108–117 (1976).
[CrossRef] [PubMed]

Other (3)

M. R. Melamed, P. F. Mullaney, M. L. Mendelsohn, eds., Flow Cytometry and Sorting (Wiley, New York, 1979), Chap. 21, p. 383.

M. Kerker, The Scattering of Light and Other Electromagnetic Radiation (Academic, New York, 1969), Chap. 7, p. 311.

C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, Amsterdam, 1983), Chap. 13, p. 381.

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Figures (2)

Fig. 1
Fig. 1

Schematic of the optical layout of the laser flow cytometer: CRO, oscilloscope; a, lens diameter; f, lens focal length.

Fig. 2
Fig. 2

Forward scatter signal detected by the photocell: (a) background resulting from the sheath; (b) signal in the presence of the 5-μm latex spheres.

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