Abstract

An optical tomography system is developed for generating three-dimensional reconstructions of thick objects from projections. The system is useful for studying transparent structures that are 1–10 mm in diameter. Evaluation of the reconstruction system with a test object demonstrates 98% geometric accuracy, 90% accuracy in the detection of boundaries, and 90% accuracy in the measurement of absorbance. Reconstructions are computed from 96 parallel projections spaced evenly within 180°. Accurate alignment of the projections is achieved with a cross-correlation method following data acquisition. Application of the optical tomography reconstruction technique to an intact cochlea permits measurement of internal structures with 16-μm pixels and a diffraction-limited resolution of 24 μm.

© 1992 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Three-dimensional imaging of single isolated cell nuclei using optical projection tomography

Mark Fauver, Eric J. Seibel, J. Richard Rahn, Michael G. Meyer, Florence W. Patten, Thomas Neumann, and Alan C. Nelson
Opt. Express 13(11) 4210-4223 (2005)

Three-dimensional optical tomography: resolution in small-object imaging

Hamid Dehghani, Brian W. Pogue, Jiang Shudong, Ben Brooksby, and Keith D. Paulsen
Appl. Opt. 42(16) 3117-3128 (2003)

Tomographic image reconstruction from optical projections in light-diffusing media

S. B. Colak, D. G. Papaioannou, G. W. ’t Hooft, M. B. van der Mark, H. Schomberg, J. C. J. Paasschens, J. B. M. Melissen, and N. A. A. J. van Asten
Appl. Opt. 36(1) 180-213 (1997)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription