Abstract

This paper presents the prototype of a hypodermic fiber-optics microscope. The instrument permits the researcher to observe tissues beneath the skin of the living animal or human, microscopically, through a hypodermic needle. The device utilizes fiber optics for image transmission, making it possible to observe through needles with inside diameters of 0.81 mm. Special features of this device include a self-contained illumination system within the same needle as the imaging bundle, and a unique microscope eyepiece which corrects for distortion caused by the necessary bevel at the far end of the needle. The resolving power of the present system is about 10 μ. A major problem is the definition of structures from each other within living tissue. Experiments are under way with various vital stains to improve this definition. Successful observations to date have been made of blood vessels and muscle fibers. Possible applications of the instrument are discussed.

© 1964 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
    [CrossRef]
  2. N. S. Kapany, J. A. Eyer, R. E. Keim, J. Opt. Soc. Am. 47, 423 (1957).
    [CrossRef]
  3. J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

1964 (1)

J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

1961 (1)

D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
[CrossRef]

1957 (1)

Capellaro, D. F.

D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
[CrossRef]

Eyer, J. A.

Goldman, J.

J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

Hornby, P.

J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

Kapany, N. S.

D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
[CrossRef]

N. S. Kapany, J. A. Eyer, R. E. Keim, J. Opt. Soc. Am. 47, 423 (1957).
[CrossRef]

Keim, R. E.

Long, C.

J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
[CrossRef]

J. Investigative Derm. (1)

J. Goldman, P. Hornby, C. Long, J. Investigative Derm. 42, 231 (1964).

J. Opt. Soc. Am. (1)

Nature (1)

D. F. Capellaro, N. S. Kapany, C. Long, Nature 191, 927 (1961).
[CrossRef]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

The hypodermic microscope in position for use.

Fig. 2
Fig. 2

Cross-section view of the probe housing.

Fig. 3
Fig. 3

View of tissue through hypodermic microscope; field is 500 μ × 700 μ. Dark streak is 90-μ blood vessel in living rat muscle. Elliptical shape is produced by crossed cylindrical lenses in eyepiece, correcting distortion due to needle bevel. Methylene blue stain.

Metrics