Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Ray tracing analysis of the image quality of a high collection efficiency mirror system

Not Accessible

Your library or personal account may give you access

Abstract

Recently, a high collection efficiency mirror system was developed by Watson [ Cytometry 10, 681– 688 ( 1989)] to increase the sensitivity of low level fluorescence detection. The mirror system consists of an ellipsoidal imaging mirror and spherical backreflecting mirror. The fluorescing sample is located at one focus of the ellipsoid, and its image is formed at the other focus. In this paper we evaluate the image quality of this geometry using a PC-based ray tracing program. The analysis demonstrates high collection efficiency but poor image quality. The effect of poor image quality on single molecule detection is discussed.

© 1990 Optical Society of America

Full Article  |  PDF Article
More Like This
Single-molecule detection with axial flow into a micrometer-sized capillary

David A. Ball, Guoqing Shen, and Lloyd M. Davis
Appl. Opt. 46(7) 1157-1164 (2007)

Rapid and efficient detection of single chromophore molecules in aqueous solution

Li-Qiang Li and Lloyd M. Davis
Appl. Opt. 34(18) 3208-3217 (1995)

Highly efficient optical detection of surface-generated fluorescence

Jörg Enderlein, Thomas Ruckstuhl, and Stefan Seeger
Appl. Opt. 38(4) 724-732 (1999)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved