The computed tomography (CT) technique is implemented in an optical transmission microscope with a laser as a light source. A rotational Pechan prism is used in the illumination optics of the microscope for projecting a thick 3-D sample onto a 2-D array detector in different directions. A minicomputer is employed for 3-D reconstruction from the observed image data. Energy throughput efficiency and signal-to-noise ratio in the developed CT microscope are higher than those of our previously developed CT microscope [ J. Opt. Soc. Am. A 4, 292 ( 1987)]. Instrumentation of this microscope is described and some experimental results are shown with biological samples.
© 1990 Optical Society of AmericaFull Article | PDF Article
S. Kawata, O. Nakamura, and S. Minami
J. Opt. Soc. Am. A 4(1) 292-297 (1987)
O. Nakamura, S. Kawata, and S. Minami
J. Opt. Soc. Am. A 5(4) 554-561 (1988)
Osamu Nakamura, Satoshi Kawata, and Shigeo Minami
Appl. Opt. 29(11) 1671-1674 (1990)