Abstract

Computer-software-controlled placement of cells onto microscope slides is advantageous for quantitative optical microscopy. Cell detection, identification, and placement have been implemented on a software-controlled flow sorter. Computer hardware timing latencies that comprise the dead times of the system are sufficiently short (10 μs) to allow adequate delay of cells for sorting. Operating system timing latencies (150 μs) are also short enough for low flow-rate sorting. As a result of the low cost of computers and because formats for cell placement are still controversial, software control is desirable since it provides the flexibility to implement multiple variations for cell detection, identification, and sorting.

© 1987 Optical Society of America

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