Abstract

A personal computer-based microphotometry system is described which provides objective measurements of important diagnostic properties of histologic specimens. Characteristic features of individual nuclei such as total integrated nuclear optical density (DNA content), nuclear area, shape, and texture may be measured. When operated at reduced magnification, measurements of mitotic density, nuclear crowding, and relative nuclear orientation provide additional diagnostic information. These features may be examined in the context provided by other nuclei and, in tissues such as stratified epithelia, relative to nuclear position within the histologic structure as a whole.

© 1987 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
High performance pathology workstation using an automated multispectral microscope

Harvey L. Kasdan, Kenneth C. Langford, John Liberty, Michael Zachariash, and Fred H. Deindoerfer
Appl. Opt. 26(16) 3294-3300 (1987)

Accuracy of optical density measurement of cells. 1: Low resolution

A. W. M. Smeulders and T. K. ten Kate
Appl. Opt. 26(16) 3249-3257 (1987)

Microcomputer-based image processing workstations for cytology

Thomas L. Hall, Darius H. L. Keyani, and Dorothy L. Rosenthal
Appl. Opt. 26(16) 3266-3269 (1987)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription