Abstract

The AMAC IIIS electrooptical transducer for flow cytometry facilitates simultaneous measurements of electrical impedance and several optical properties of single cells. Ray-trace analysis demonstrates that its spherical exterior permits light rays to emerge effectively undeviated and thus lowers the required numerical aperture of the collecting lens as compared with a flat surfaced transducer. This feature results in an increased light-gathering ability, an increased depth of focus, minimum spherical and chromatic aberrations, and minimizes the optical volume observed.

© 1987 Optical Society of America

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