The construction and preliminary application of a thin-layer optical cell suitable for measuring absorption and fluorescence of highly absorbent solutions are described. The light path length is easily variable down to 2.5 μm with a commercial torque driver. Both absorption and fluorescence are measurable in a cell by conventional spectrophotometers and spectrofluorophotometers. The emission spectra of concentrated rhodamine B aqueous solutions up to 15 mM were measured with the thin-layer cell. The thin-layer cell showed great improvement in concentration quenching because of its very short light path length.
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