Abstract

Using laser light scattering a rapid method for estimating relative counts of less deformable erythrocytes in blood samples was developed. The procedure is based on comparison of light scattering from blood cells deformed in defined shear stress and blood cells at rest, respectively.

© 1982 Optical Society of America

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References

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  1. P. Teitel, Nouv. Rev. Fr. Hematol. 7, 195 (1967).
    [PubMed]
  2. R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
    [CrossRef] [PubMed]
  3. R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
    [CrossRef]
  4. R. E. Wells, H. Schmid-Schönbein, J. Appl. Physiol. 27, 213 (1969).
    [PubMed]
  5. S. S. Bull, J. D. Brailsford, Blood 45, 581 (1975).
    [PubMed]
  6. W. D. Corry, H. J. Meiselman, Blood 51, 693 (1978).
    [PubMed]
  7. M. Bessis, N. Mohandas, Blood Cells 1, 307, (1975).
  8. W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).
  9. M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
    [PubMed]
  10. T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).
  11. H. C. van de Hulst, Light Scattering by Small Particles (Wiley, New York, 1957).
  12. F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
    [CrossRef]
  13. M. Bessis, “Corpuscles,” in Atlas of Red Blood Cell Shapes (Springer, Berlin, 1974).

1980 (3)

W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).

M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
[PubMed]

F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
[CrossRef]

1978 (1)

W. D. Corry, H. J. Meiselman, Blood 51, 693 (1978).
[PubMed]

1976 (1)

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

1975 (2)

S. S. Bull, J. D. Brailsford, Blood 45, 581 (1975).
[PubMed]

M. Bessis, N. Mohandas, Blood Cells 1, 307, (1975).

1969 (2)

R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
[CrossRef]

R. E. Wells, H. Schmid-Schönbein, J. Appl. Physiol. 27, 213 (1969).
[PubMed]

1967 (1)

P. Teitel, Nouv. Rev. Fr. Hematol. 7, 195 (1967).
[PubMed]

Barnes, A. J.

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Bessis, M.

W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).

M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
[PubMed]

M. Bessis, N. Mohandas, Blood Cells 1, 307, (1975).

M. Bessis, “Corpuscles,” in Atlas of Red Blood Cell Shapes (Springer, Berlin, 1974).

Blazek, V.

F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
[CrossRef]

Brabec, V.

T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).

Brailsford, J. D.

S. S. Bull, J. D. Brailsford, Blood 45, 581 (1975).
[PubMed]

Bull, S. S.

S. S. Bull, J. D. Brailsford, Blood 45, 581 (1975).
[PubMed]

Corry, W. D.

W. D. Corry, H. J. Meiselman, Blood 51, 693 (1978).
[PubMed]

Dormandy, J. A.

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Dormandy, T. L.

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Feo, C.

M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
[PubMed]

Groner, W.

W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).

La Celle, P. L.

R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
[CrossRef]

Lock, P. J.

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Marik, T.

T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).

Meiselman, H. J.

W. D. Corry, H. J. Meiselman, Blood 51, 693 (1978).
[PubMed]

Merril, E. W.

R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
[CrossRef]

Mohandas, N.

W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).

M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
[PubMed]

M. Bessis, N. Mohandas, Blood Cells 1, 307, (1975).

Muzik, J.

F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
[CrossRef]

T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).

Plasek, J.

T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).

Reid, R. L.

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Schmid-Schönbein, H.

R. E. Wells, H. Schmid-Schönbein, J. Appl. Physiol. 27, 213 (1969).
[PubMed]

Stozicky, F.

F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
[CrossRef]

Teitel, P.

P. Teitel, Nouv. Rev. Fr. Hematol. 7, 195 (1967).
[PubMed]

van de Hulst, H. C.

H. C. van de Hulst, Light Scattering by Small Particles (Wiley, New York, 1957).

Wells, R. E.

R. E. Wells, H. Schmid-Schönbein, J. Appl. Physiol. 27, 213 (1969).
[PubMed]

Wood, R. I.

R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
[CrossRef]

Blood (2)

S. S. Bull, J. D. Brailsford, Blood 45, 581 (1975).
[PubMed]

W. D. Corry, H. J. Meiselman, Blood 51, 693 (1978).
[PubMed]

Blood Cells (2)

M. Bessis, N. Mohandas, Blood Cells 1, 307, (1975).

M. Bessis, N. Mohandas, C. Feo, Blood Cells 6, 315 (1980).
[PubMed]

Clin. Chem. N.Y. (1)

W. Groner, N. Mohandas, M. Bessis, Clin. Chem. N.Y. 26, 1435 (1980).

J. Appl. Physiol. (1)

R. E. Wells, H. Schmid-Schönbein, J. Appl. Physiol. 27, 213 (1969).
[PubMed]

J. Biomech. (1)

F. Stozicky, V. Blazek, J. Muzik, J. Biomech. 13, 417 (1980).
[CrossRef]

J. Clin. Invest. (1)

R. I. Wood, P. L. La Celle, E. W. Merril, J. Clin. Invest. 48, 795 (1969).
[CrossRef]

J. Clin. Pathol. (1)

R. L. Reid, A. J. Barnes, P. J. Lock, J. A. Dormandy, T. L. Dormandy, J. Clin. Pathol. 29, 855 (1976).
[CrossRef] [PubMed]

Nouv. Rev. Fr. Hematol. (1)

P. Teitel, Nouv. Rev. Fr. Hematol. 7, 195 (1967).
[PubMed]

Other (3)

M. Bessis, “Corpuscles,” in Atlas of Red Blood Cell Shapes (Springer, Berlin, 1974).

T. Marik, J. Plasek, V. Brabec, J. Muzik, Clin. Hemorheology (in press).

H. C. van de Hulst, Light Scattering by Small Particles (Wiley, New York, 1957).

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Figures (5)

Fig. 1
Fig. 1

Schematic diagram of ektacytometer.

Fig. 2
Fig. 2

Outline of diffraction patterns and schematic diagram of scattered light intensity measurement.

Fig. 3
Fig. 3

Dependence of scattered light intensity on erythrocyte concentration.

Fig. 4
Fig. 4

Dependence of (A) elliptical diffraction pattern dimensions and (B) Is scattered light intensity on applied shear stress.

Fig. 5
Fig. 5

Dependence of undeformable erythrocyte index I U m the concentration of glutaraldehyde-fixed erythrocytes.

Tables (1)

Tables Icon

Table I Intensities of Scattered Light from Various Components of Blood Suspensions

Equations (7)

Equations on this page are rendered with MathJax. Learn more.

I   =   ( K D N D +   K U N U ) I i + Δ ,
I S   =   K U S N U I i + Δ S ,
I 0 = ( K D 0 N D + K U 0 N U ) I i + Δ 0 .
I U = I S Δ S ( I 0 Δ 0 ) ( I S Δ S ) ,
I U = K U S N U ( K D 0 N D + K U 0 K U S ) N U .
K U 0 K U S ,
I U K U S K D 0 N U N D .

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