Abstract

Microspectrophotometers, manual and recording, have been constructed with cadmium selenide (CdSe) photoconductive cells and are in use in our laboratory as a spectroscopic tool in the study of the pigment chemistry of photoreceptors in living cells. The basic design and operation of these microspectrophotometers for obtaining absorption spectra are described. The absorption spectrum from the ultraviolet, visible, and near infrared can be obtained in a single sweep for specimen areas as small as 2 μ2. Examples of absorption spectra of the chloroplast of plant cells, the retinal rods of the visual cells of the eye, and the red blood cell are illustrated. The applications of microspectrophotometry to a variety of biological, biochemical, and chemical problems are indicated.

© 1963 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Structure and Molecular Organization of Retinal Photoreceptors*†

Jerome J. Wolken
J. Opt. Soc. Am. 53(1) 1-19 (1963)

Spectral Properties of Plants

David M. Gates, Harry J. Keegan, John C. Schleter, and Victor R. Weidner
Appl. Opt. 4(1) 11-20 (1965)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription