Abstract

A special mathematical model is developed to find the scattered light pattern of biological cells in a fluid flow system. A paraxial approximation to the diffraction integral is used to accommodate nonspherical and asymmetrical cells. The paraxial approximation results are in good agreement with Mie calculations for homogeneous spheres of comparable size and refractive index. A 2-D fast Fourier transform program is used to simulate variable cell morphology and experimental conditions. Distinct signature differences for two types of cells resembling normal and abnormal malignant human cervical cells are found useful as discriminant features. The ability to handle a variety of model cell shapes and distortions by these methods is demonstrated, and use of signature features for cell discrimination is shown experimentally.

© 1978 Optical Society of America

Fourier optical approach to the extraction of morphological parameters from the diffraction pattern of biological cells

Bernhard Türke, Günter Seger, Manfred Achatz, and Werner v. Seelen
Appl. Opt. 17(17) 2754-2761 (1978)

Screening of cervical cytological samples using coherent optical processing. Part 1

B. Pernick, R. E. Kopp, J. Lisa, J. Mendelsohn, H. Stone, and R. Wohlers
Appl. Opt. 17(1) 21-34 (1978)

Screening of cervical cytological samples using coherent optical processing. Part 2

R. Wohlers, J. Mendelsohn, R. E. Kopp, and B. J. Pernick
Appl. Opt. 17(1) 35-42 (1978)

Light Scattering from Coated Spheres: Model for Biological Cells

A. Brunsting and P. F. Mullaney
Appl. Opt. 11(3) 675-680 (1972)

Elastic light scattering from nucleated blood cells: rapid numerical analysis

P. M. A. Sloot and C. G. Figdor
Appl. Opt. 25(19) 3559-3565 (1986)