Abstract

A Q-switched ruby laser was used to irradiate excised biological tissues. An isolated narrow band emission line was observed at 347 nm on irradiation of collagenous tissues. Temporal pulses at 347 nm were narrower than the laser pulses at 694 nm. Reduced crystalline glutathione yielded narrow band emission at 347 nm that was similar to that for the collagenous tissues. The observed narrow band emission at 347 nm is thought to be due to optical second harmonic generation.

© 1971 Optical Society of America

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References

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  1. S. Fine, Federation Proc. 24, Suppl. 14, S47 (1965).
  2. M. M. Zaret, Federation Proc. 24, Suppl. 14, S62 (1965).
  3. K. E. Reickhoff, W. J. Peticolas, Science 147, 610 (1965).
    [CrossRef]
  4. L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].
  5. B. A. Lengyel, Introduction to Laser Physics (Wiley, New York, 1966), p. 101.
  6. V. P. Chebotaev, L. S. Vasilenko, Zh. Eksp. Teor. Fiz. 48, 779 (1965) [Sov. Phys. JETP 21, 515 (1965)].
  7. P. A. Franken, J. F. Ward, Rev. Mod. Phys. 35, 23 (1963).
    [CrossRef]

1965 (5)

S. Fine, Federation Proc. 24, Suppl. 14, S47 (1965).

M. M. Zaret, Federation Proc. 24, Suppl. 14, S62 (1965).

K. E. Reickhoff, W. J. Peticolas, Science 147, 610 (1965).
[CrossRef]

L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].

V. P. Chebotaev, L. S. Vasilenko, Zh. Eksp. Teor. Fiz. 48, 779 (1965) [Sov. Phys. JETP 21, 515 (1965)].

1963 (1)

P. A. Franken, J. F. Ward, Rev. Mod. Phys. 35, 23 (1963).
[CrossRef]

Chebotaev, V. P.

L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].

V. P. Chebotaev, L. S. Vasilenko, Zh. Eksp. Teor. Fiz. 48, 779 (1965) [Sov. Phys. JETP 21, 515 (1965)].

Fine, S.

S. Fine, Federation Proc. 24, Suppl. 14, S47 (1965).

Franken, P. A.

P. A. Franken, J. F. Ward, Rev. Mod. Phys. 35, 23 (1963).
[CrossRef]

Lengyel, B. A.

B. A. Lengyel, Introduction to Laser Physics (Wiley, New York, 1966), p. 101.

Peticolas, W. J.

K. E. Reickhoff, W. J. Peticolas, Science 147, 610 (1965).
[CrossRef]

Reickhoff, K. E.

K. E. Reickhoff, W. J. Peticolas, Science 147, 610 (1965).
[CrossRef]

Troitskii, Yu. V.

L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].

Vasilenko, L. S.

L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].

V. P. Chebotaev, L. S. Vasilenko, Zh. Eksp. Teor. Fiz. 48, 779 (1965) [Sov. Phys. JETP 21, 515 (1965)].

Ward, J. F.

P. A. Franken, J. F. Ward, Rev. Mod. Phys. 35, 23 (1963).
[CrossRef]

Zaret, M. M.

M. M. Zaret, Federation Proc. 24, Suppl. 14, S62 (1965).

Federation Proc. (2)

S. Fine, Federation Proc. 24, Suppl. 14, S47 (1965).

M. M. Zaret, Federation Proc. 24, Suppl. 14, S62 (1965).

Rev. Mod. Phys. (1)

P. A. Franken, J. F. Ward, Rev. Mod. Phys. 35, 23 (1963).
[CrossRef]

Science (1)

K. E. Reickhoff, W. J. Peticolas, Science 147, 610 (1965).
[CrossRef]

Zh. Eksp. Teor. Fiz. (2)

L. S. Vasilenko, V. P. Chebotaev, Yu. V. Troitskii, Zh. Eksp. Teor. Fiz. 48, 777 (1965) [Sov. Phys. JETP 21, 513 (1965)].

V. P. Chebotaev, L. S. Vasilenko, Zh. Eksp. Teor. Fiz. 48, 779 (1965) [Sov. Phys. JETP 21, 515 (1965)].

Other (1)

B. A. Lengyel, Introduction to Laser Physics (Wiley, New York, 1966), p. 101.

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Figures (3)

Fig. 1
Fig. 1

Schematic of experimental apparatus.

Fig. 2
Fig. 2

Example of moderate resolution emission spectrum from dog cornea. Similar spectra were observed from sclera, tendon, skin, and glutathione on irradiation at 694 nm. With narrower slits the emission linewidth from corneas was found to be less than one-half that depicted here. Response of 1P28 photomultiplier–CuSO4 filter combination is shown normalized to unity near the wavelength of maximum response. Slit width was increased to yield an approximate 25-nm linewidth resolution over the complete spectral range for rapid scanning. Error bars are shown at the peak emission wavelength for the probable error in emission amplitude and for the monochromator wavelength scale setting accuracy. Response of the photomultiplier is from the manufactuier’s data. CuSO4 transmission was measured in a spectrophotometer.

Fig. 3
Fig. 3

Drawing of normalized laser and sample emission pulses taken from photograph of dual-beam oscilloscope traces. Full width at half-maximum for the laser pulse is 97 nsec, while it is 63 nsec for the emission pulse.

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