Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Statistical precision in super-resolution optical fluctuation imaging

Not Accessible

Your library or personal account may give you access

Abstract

The super-resolution optical fluctuation imaging (SOFI) technique enhances image spatial resolution by calculating the spatiotemporal cross-cumulants of independent stochastic intensity fluctuations of emitters. Ideally, SOFI eliminates any noise that is not correlated over time, but in practice, due to limited data lengths, the statistical uncertainty of cumulants will affect the continuities and homogeneities of SOFI images. Since the variance and signal-to-noise ratio (SNR) characterize cumulant statistical uncertainty, we determined theoretical expressions for these based on a single dataset. From a simulation of temporal fluctuations of blinking fluorescent emitters, we calculated the quantitative relation between the SNR of cumulants and multiple parameters of the blinking signal, such as the on-time ratio, acquisition frame to average blinking rate ratio, sequence length, and photon amplitude, which not only provides a physical interpretation for SOFI phenomena but also theoretical guidance to achieve optimal practical outcomes.

© 2016 Optical Society of America

Full Article  |  PDF Article
More Like This
Model-free uncertainty estimation in stochastical optical fluctuation imaging (SOFI) leads to a doubled temporal resolution

Wim Vandenberg, Sam Duwé, Marcel Leutenegger, Benjamien Moeyaert, Bartosz Krajnik, Theo Lasser, and Peter Dedecker
Biomed. Opt. Express 7(2) 467-480 (2016)

Fourier interpolation stochastic optical fluctuation imaging

Simon C. Stein, Anja Huss, Dirk Hähnel, Ingo Gregor, and Jörg Enderlein
Opt. Express 23(12) 16154-16163 (2015)

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Wim Vandenberg, Marcel Leutenegger, Sam Duwé, and Peter Dedecker
Opt. Express 27(18) 25749-25766 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved