Abstract

Spectral domain phase microscopy (SDPM) has been reported in the literature as a functional extension to low-coherence interferometry, which enables nanoscale measurement of a scatter’s displacement. The signal in SDPM is generated from structural images that lack molecular specificity. This paper investigates the expansion of phase analysis to fluorescence self-interference signals to provide functional information about a sample. Spectral domain fluorescence coherence phase microscopy is demonstrated for nanoscale resolution motion detection of fluorescent particles with a signal-to-noise ratio limited resolution of ~10 nm. This paper demonstrates the feasibility of combining phase processing with fluorescence self-interference, which may be useful for future applications such as cell rheology.

© 2011 Optical Society of America

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    [CrossRef]
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    [CrossRef]
  5. K. H. Drexhage, “IV interaction of light with monomolecular dye layers,” in Progress in Optics, E.Wolf, ed. (Elsevier, 1974), pp. 163–232.
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2010 (1)

2007 (2)

A. K. Ellerbee and J. A. Izatt, “Phase retrieval in low-coherence interferometric microscopy,” Opt. Lett. 32, 388–390 (2007).
[CrossRef]

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

2006 (2)

2005 (2)

2003 (2)

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

2002 (1)

1997 (1)

D. Braun and P. Fromherz, “Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon,” Appl. Phys. A 65, 341–348 (1997).
[CrossRef]

1989 (1)

K. E. Drabe, G. Cnossen, and D. A. Wiersma, “Localization of spontaneous emission in front of a mirror,” Opt. Commun. 73, 91–95 (1989).
[CrossRef]

Aguet, F.

Akkin, T.

Applegate, B. E.

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

Bilenca, A.

Bocchio, N. L.

Bouma, B.

Braun, D.

D. Braun and P. Fromherz, “Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon,” Appl. Phys. A 65, 341–348 (1997).
[CrossRef]

Cantor, C. R.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Cense, B.

Chance, R. R.

R. R. Chance, A. Prock, and R. Silbey, “Molecular fluorescence and energy transfer near interfaces,” in Advances in Chemical Physics (Wiley, 1978), pp. 1–65.

Choma, M. A.

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

M. A. Choma, A. K. Ellerbee, C. Yang, T. L. Creazzo, and J. A. Izatt, “Spectral-domain phase microscopy,” Opt. Lett. 30, 1162–1164 (2005).
[CrossRef]

Cnossen, G.

K. E. Drabe, G. Cnossen, and D. A. Wiersma, “Localization of spontaneous emission in front of a mirror,” Opt. Commun. 73, 91–95 (1989).
[CrossRef]

Creazzo, T. L.

Davis, B.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

de Boer, J. F.

Drabe, K. E.

K. E. Drabe, G. Cnossen, and D. A. Wiersma, “Localization of spontaneous emission in front of a mirror,” Opt. Commun. 73, 91–95 (1989).
[CrossRef]

Drexhage, K. H.

K. H. Drexhage, “IV interaction of light with monomolecular dye layers,” in Progress in Optics, E.Wolf, ed. (Elsevier, 1974), pp. 163–232.

Drexler, W.

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

Ellerbee, A. K.

A. K. Ellerbee and J. A. Izatt, “Phase retrieval in low-coherence interferometric microscopy,” Opt. Lett. 32, 388–390 (2007).
[CrossRef]

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

M. A. Choma, A. K. Ellerbee, C. Yang, T. L. Creazzo, and J. A. Izatt, “Spectral-domain phase microscopy,” Opt. Lett. 30, 1162–1164 (2005).
[CrossRef]

Fercher, A. F.

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

Fromherz, P.

A. Lambacher and P. Fromherz, “Luminescence of dye molecules on oxidized silicon and fluorescence interference contrast microscopy of biomembranes,” J. Opt. Soc. Am. B 19, 1435–1453 (2002).
[CrossRef]

D. Braun and P. Fromherz, “Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon,” Appl. Phys. A 65, 341–348 (1997).
[CrossRef]

Geissbuehler, S.

Goldberg, B. B.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Hitzenberger, C. K.

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

Ippolito, S. B.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Izatt, J. A.

Joo, C.

Karl, W. C.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Lambacher, A.

Lasser, T.

I. Märki, N. L. Bocchio, S. Geissbuehler, F. Aguet, A. Bilenca, and T. Lasser, “Three-dimensional nano-localization of single fluorescent emitters,” Opt. Express 18, 20263–20272(2010).
[CrossRef]

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

Märki, I.

McDowell, E. J.

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

Moiseev, L. A.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Ozcan, A.

Park, B. H.

Prock, A.

R. R. Chance, A. Prock, and R. Silbey, “Molecular fluorescence and energy transfer near interfaces,” in Advances in Chemical Physics (Wiley, 1978), pp. 1–65.

Sarunic, M. V.

Silbey, R.

R. R. Chance, A. Prock, and R. Silbey, “Molecular fluorescence and energy transfer near interfaces,” in Advances in Chemical Physics (Wiley, 1978), pp. 1–65.

Swan, A. K.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Tearney, G.

Unlu, M. S.

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

Weinberg, S.

Wiersma, D. A.

K. E. Drabe, G. Cnossen, and D. A. Wiersma, “Localization of spontaneous emission in front of a mirror,” Opt. Commun. 73, 91–95 (1989).
[CrossRef]

Yang, C.

Appl. Phys. A (1)

D. Braun and P. Fromherz, “Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon,” Appl. Phys. A 65, 341–348 (1997).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron. (1)

A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, and M. S. Unlu, “Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference,” IEEE J. Sel. Top. Quantum Electron. 9, 294–300 (2003).
[CrossRef]

J. Biomed. Opt. (1)

E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, “Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells,” J. Biomed. Opt. 12, 044008 (2007).
[CrossRef]

J. Opt. Soc. Am. B (1)

Opt. Commun. (1)

K. E. Drabe, G. Cnossen, and D. A. Wiersma, “Localization of spontaneous emission in front of a mirror,” Opt. Commun. 73, 91–95 (1989).
[CrossRef]

Opt. Express (2)

Opt. Lett. (4)

Rep. Prog. Phys. (1)

A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography—principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
[CrossRef]

Other (2)

K. H. Drexhage, “IV interaction of light with monomolecular dye layers,” in Progress in Optics, E.Wolf, ed. (Elsevier, 1974), pp. 163–232.

R. R. Chance, A. Prock, and R. Silbey, “Molecular fluorescence and energy transfer near interfaces,” in Advances in Chemical Physics (Wiley, 1978), pp. 1–65.

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