Abstract
Adsorption of the elongated human plasma fibrinogen (HPF) and globular human serum albumin molecules on a titanium-based surface is monitored by analyzing permittivity and optical roughness of protein-modified surfaces by using a diffractive optical element (DOE)-based sensor and variable angle spectro-ellipsometry (VASE). Both DOE and VASE confirmed that fibrinogen forms a thicker and more packed surface adlayer compared to a more porous and weakly adsorbed albumin adlayer. A linear relation of the permittivity () and dielectric loss () was found for some of the dry titanium-doped hydrocarbon (TDHC) surfaces with excellent HPF adsorption ability. We discuss some aspects of TDHC’s aging and its possible effects on fibrinogen adsorption.
© 2010 Optical Society of America
Full Article | PDF ArticleMore Like This
Raimo Silvennoinen, Vladimir Vetterl, Stanislav Hasoň, Heikki Tuononen, Martti Silvennoinen, Kari Myller, Ladislav Cvrček, Jiři Vanek, and Patrik Prachăr
Opt. Express 16(14) 10130-10140 (2008)
Niko Penttinen, Stanislav Hasoň, Jiři Vaněk, and Raimo Silvennoinen
Appl. Opt. 51(10) C32-C37 (2012)
Tiago A. T. Sousa, Leiva C. Oliveira, Franz H. Neff, Hervé M. Laborde, and Antonio M. N. Lima
Appl. Opt. 57(24) 6866-6875 (2018)