Abstract

We report on the design and construction of a confocal light scattering spectroscopic imaging system aimed ultimately to conduct depth-resolved characterization of biological tissues. The confocal sectioning ability of the system is demonstrated using a two-layer sample consisting of a 200μm thick cancer cell layer on top of a scattering layer doped with a green absorber. The measurement results demonstrate that distinct light scattering signals can be isolated from each layer with an axial and a lateral resolution of 30 and 27μm, respectively. Such a system is expected to have significant applications in the areas of tissue engineering and disease diagnostics and monitoring.

© 2009 Optical Society of America

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