Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250–1100 nm dependent on concentration

Abstract

The real part of the complex refractive index of oxygenated native hemoglobin solutions dependent on concentration was determined in the wavelength range 250 to $1100\text{\hspace{0.17em} nm}$ by Fresnel reflectance measurements. The hemoglobin solution was produced by physical hemolysis of human erythrocytes followed by ultracentrifugation and filtration. A model function is presented for calculating the refractive index of hemoglobin solutions depending on concentration in the wavelength range 250 to $1100\text{\hspace{0.17em} nm}$.

© 2006 Optical Society of America