Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Excitation-emission fluorimeter based on linear interference filters

Not Accessible

Your library or personal account may give you access

Abstract

We describe the design, properties, and performance of an excitation-emission (EE) fluorimeter that enables spectral characterization of an object simultaneously with respect to both its excitation and its emission properties. Such devices require two wavelength-selecting elements, one in the optical path of the excitation broadband light to obtain tunable excitation and the other to analyze the resulting fluorescence. Existing EE instruments are usually implemented with two monochromators. The key feature of our EE fluorimeter is that it employs lightweight and compact linear interference filters (LIFs) as the wavelength-selection elements. The spectral tuning of both the excitation and the detection LIFs is achieved by their mechanical shift relative to each other by use of two computer-controlled linear step motors. The performance of the LIF-based EE fluorimeter is demonstrated with the fluorescent spectra of various dyes and their mixtures.

© 2004 Optical Society of America

Full Article  |  PDF Article
More Like This
Rapid multiexcitation fluorescence spectroscopy system for in vivo tissue diagnosis

Renato Amaro Zângaro, Landulfo Silveira, Ramasamy Manoharan, George Zonios, Irving Itzkan, Ramachandra R. Dasari, Jacques Van Dam, and Michael S. Feld
Appl. Opt. 35(25) 5211-5219 (1996)

Excitation nonlinearities in emission reabsorption laser-induced fluorescence techniques

Carlos H. Hidrovo, Ricardo R. Brau, and Douglas P. Hart
Appl. Opt. 43(4) 894-913 (2004)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved