Abstract

A first-order thermal analysis is applied to Laser Capture Microdissection (LCM), a new microscope technique for routine targeting and extraction of specific cells from tissue sections for subsequent multiplex molecular analysis. In LCM a polymer film placed in contact with the tissue is focally activated by a pulsed IR laser beam and is melted and bonded to adjacent targeted cells. A three-dimensional finite-element model is used to predict the thermal transients within the polymer, the captured tissue, and its macromolecules. The simulations allow a comparison of models for the physical process of LCM with the experimental data on the dependence of the transfer spot size on laser power. The validated physical model and the thermal simulations permit optimization of the complex LCM parameter space for a wide variety of configurations and applications.

© 1998 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Temperature control during laser vessel welding

T. A. Springer and A. J. Welch
Appl. Opt. 32(4) 517-525 (1993)

Thermal response of a dental tissue induced by femtosecond laser pulses

K. P. Chang, T. W. Tsai, K. Y. Huang, C. H. Huang, S. Y. Wang, C. W. Cheng, J. K. Chen, and D. Y. Tzou
Appl. Opt. 52(27) 6626-6635 (2013)

Femtosecond laser photo-response of Ge23Sb7S70 films

Troy Anderson, Laeticia Petit, Nathan Carlie, Jiyeon Choi, Juejun Hu, Anu Agarwal, Lionel Kimerling, Kathleen Richardson, and Martin Richardson
Opt. Express 16(24) 20081-20098 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (12)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription