Abstract

In the field of biology there are many problems that call for microscopic image analysis. Prominent among these is the study of the change of shape and of the motion of single cells. Such changes are difficult to analyze in real time; taking series of photographs, for example, and evaluating these photographs by microdensitometry and subsequent computer analysis are rather time-consuming processes. We present here another solution to this problem, using equipment that should be available even in the modestly equipped laboratory: a microscope, a scanning system such as a video monitor, and a microcomputer that can be interfaced with the scanner. The necessary procedures of data acquisition and data processing are discussed in detail.

© 1983 Optical Society of America

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References

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  1. G. B. Olson et al., Proc. Soc. Photo-Opt. Instrum. Eng. 232, 54 (1980).
  2. G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).
  3. M. Bessis, Nouv. Rev. Fr. Hématol. 13, 285 (1973).
  4. J. P. Giroud, N. Roch-Arweiller, Biomedicine 27, 246 (1977).
    [PubMed]
  5. F. Roberts, J. Z. Young, Nature (London) 167, 231 (1951).
    [CrossRef]
  6. Hewlett-Packard, Manuel d’utilisation et Guide de programmation HP 85.
  7. A. Bijaoui, Image et Information (Editions Masson, Paris, 1981).
  8. G. Roblin, M. Prévost, Appl. Opt. 19, 2638 (1980).
    [CrossRef] [PubMed]

1980

G. B. Olson et al., Proc. Soc. Photo-Opt. Instrum. Eng. 232, 54 (1980).

G. Roblin, M. Prévost, Appl. Opt. 19, 2638 (1980).
[CrossRef] [PubMed]

1978

G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).

1977

J. P. Giroud, N. Roch-Arweiller, Biomedicine 27, 246 (1977).
[PubMed]

1973

M. Bessis, Nouv. Rev. Fr. Hématol. 13, 285 (1973).

1951

F. Roberts, J. Z. Young, Nature (London) 167, 231 (1951).
[CrossRef]

Bessis, M.

M. Bessis, Nouv. Rev. Fr. Hématol. 13, 285 (1973).

Bijaoui, A.

A. Bijaoui, Image et Information (Editions Masson, Paris, 1981).

Giroud, J. P.

J. P. Giroud, N. Roch-Arweiller, Biomedicine 27, 246 (1977).
[PubMed]

Hunger, R.

G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).

Olson, G. B.

G. B. Olson et al., Proc. Soc. Photo-Opt. Instrum. Eng. 232, 54 (1980).

Prévost, M.

Roberts, F.

F. Roberts, J. Z. Young, Nature (London) 167, 231 (1951).
[CrossRef]

Roblin, G.

Roch-Arweiller, N.

J. P. Giroud, N. Roch-Arweiller, Biomedicine 27, 246 (1977).
[PubMed]

Schett, W.

G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).

Schoeppe, G.

G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).

Young, J. Z.

F. Roberts, J. Z. Young, Nature (London) 167, 231 (1951).
[CrossRef]

Appl. Opt.

Biomedicine

J. P. Giroud, N. Roch-Arweiller, Biomedicine 27, 246 (1977).
[PubMed]

Jena Rev.

G. Schoeppe, W. Schett, R. Hunger, Jena Rev. 6, 264 (1978).

Nature (London)

F. Roberts, J. Z. Young, Nature (London) 167, 231 (1951).
[CrossRef]

Nouv. Rev. Fr. Hématol.

M. Bessis, Nouv. Rev. Fr. Hématol. 13, 285 (1973).

Proc. Soc. Photo-Opt. Instrum. Eng.

G. B. Olson et al., Proc. Soc. Photo-Opt. Instrum. Eng. 232, 54 (1980).

Other

Hewlett-Packard, Manuel d’utilisation et Guide de programmation HP 85.

A. Bijaoui, Image et Information (Editions Masson, Paris, 1981).

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Figures (6)

Fig. 1
Fig. 1

Variations du rapport de l’intervalle entre points d’analyse à la limite de résolution en fonction du nombre de points par ligne pour les objectifs de microscope les plus classiques.

Fig. 2
Fig. 2

Après comparaison à un signal de référence VR, le signal analogique de balayage VA est transformé en un signal binaire VB. Son association à un signal horloge VH est équivalente à un signal VE à l’entrèe de l’ordinateur composé d’une suite de bits 0 et 1.

Fig. 3
Fig. 3

Organigramme de l’expérience jusqu’à l’entrée de l’ordinateur.

Fig. 4
Fig. 4

Restitution de l’image binaire d’une diatomée (a) et de ses contours (b).

Fig. 5
Fig. 5

Procédure de restitution d’un contour.

Fig. 6
Fig. 6

Exemple de restitution de la trajectoire et de l’évolution de la forme.

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