Abstract

We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multiangle acquisitions on 3D cell cultures embedded in extracellular matrices. Lens-free microscopy acquisitions present some inherent issues such as the lack of phase information on the sensor plane and a limited angular coverage. We developed and compared three different algorithms based on the Fourier diffraction theorem to obtain fully 3D reconstructions. These algorithms present an increasing complexity associated with a better reconstruction quality. Two of them are based on a regularized inverse problem approach. To compare the reconstruction methods in terms of artefact reduction, signal-to-noise ratio, and computation time, we tested them on two experimental datasets: an endothelial cell culture and a prostate cell culture grown in a 3D extracellular matrix with large reconstructed volumes up to 5mm3 with a resolution sufficient to resolve isolated single cells. The lens-free reconstructions compare well with standard microscopy.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Lensfree diffractive tomography for the imaging of 3D cell cultures

F. Momey, A. Berdeu, T. Bordy, J.-M. Dinten, F. Kermarrec Marcel, N. Picollet-D’hahan, X. Gidrol, and C. Allier
Biomed. Opt. Express 7(3) 949-962 (2016)

Multispectral total-variation reconstruction applied to lens-free microscopy

L. Herve, O. Cioni, P. Blandin, F. Navarro, M. Menneteau, T. Bordy, S. Morales, and C. Allier
Biomed. Opt. Express 9(11) 5828-5836 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Supplementary Material (6)

NameDescription
Visualization 1: AVI (1102 KB)      Visualization_1_HUVEC_3D_view
Visualization 2: AVI (2021 KB)      Visualization_2_RWPE1_3D_view
Visualization 3: AVI (4036 KB)      Visualization_3_RWPE1_3D_view
Visualization 4: TIF (2512 KB)      Visualization_4_comparison_microscope_average
Visualization 5: AVI (5544 KB)      Visualization_5_comparison_microscope_z-stack
Visualization 6: TIF (11202 KB)      Visualization_6_comparison_microscope_z-stack

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (25)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics